PXD045525-1

PXD045525 is an original dataset announced via ProteomeXchange.

Title	Secreted footprint of pluripotency in Man-13 HeSCs
Description	With a view to developing a much-needed non-invasive method for monitoring the healthy pluripotent state of human stem cells in culture, we undertook proteomic analysis of the waste medium from cultured induced (Rebl.PAT) human pluripotent stem cells (hPSCs). Cells were grown in E8 medium to maintain pluripotency, and then transferred to FGF2 and TGFβ deficient E6 media for 48 hours to replicate an early, undirected dissolution of pluripotency. We identified a distinct proteomic footprint associated with early loss of pluripotency in both hPSC lines, and a strong correlation with changes in the transcriptome. We demonstrate that multiplexing of four E8- against four E6- enriched secretome biomarkers provides a robust, diagnostic metric for pluripotent state. These biomarkers were further confirmed by Western blotting which demonstrated consistent correlation with the pluripotent state across cell lines, and in response to a recovery assay.
HostingRepository	PRIDE
AnnounceDate	2024-03-26
AnnouncementXML	Submission_2024-03-26_15:18:55.471.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phil Lewis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-20 08:33:25	ID requested
⏵ 1	2024-03-26 15:18:55	announced

Dataset with its publication pending

submitter keyword: pluripotency,IPSC, stem cells

Susan Kimber
contact affiliation	University of Manchester
contact email	susan.kimber@manchester.ac.uk
lab head
Phil Lewis
contact affiliation	University of Bristol
contact email	phil.lewis@bristol.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045525
     1. Label: PRIDE project
     2. Name: Secreted footprint of pluripotency in Man-13 HeSCs