PXD045524-1

PXD045524 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cell wall proteomics in live Mycobacterium tuberculosis uncovers exposure of ESX substrates to the periplasm
Description	The cell wall of mycobacteria plays a key role in interactions with the environment and its ability to act as a selective filter is crucial to bacterial survival. Proteins in the cell wall enable this function by mediating the import and export of diverse metabolites from ions to lipids to proteins. Accurately identifying cell wall proteins is an important step in assigning function, especially as many mycobacterial proteins lack functionally characterized homologues. Current methods for protein localization have inherent limitations that reduce accuracy. Here we showed that protein tagging by the engineered peroxidase APEX2 within live Mycobacterium tuberculosis enabled the accurate identification of the cytosolic and cell wall proteomes. Our data indicate that substrates of the virulence-associated Type VII ESX secretion system are exposed to the Mtb periplasm, providing insight into the currently unknown mechanism by which these proteins cross the mycobacterial cell envelope. The mass spectrometry raw files and search results for the APEX strategy are included here.
HostingRepository	MassIVE
AnnounceDate	2023-09-20
AnnouncementXML	Submission_2023-09-20_08:51:00.030.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Beatrix Ueberheide
SpeciesList	scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
ModificationList	Oxidation; Deamidated
Instrument	Q Exactive; Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-20 08:28:48	ID requested
⏵ 1	2023-09-20 08:51:00	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Mycobacterium tuberculosis, APEX, Proximity Labeling, cytosolic and cell wall proteomes, Type VII ESX secretion system

submitter keyword: Mycobacterium tuberculosis, APEX, Proximity Labeling, cytosolic and cell wall proteomes, Type VII ESX secretion system

Contact List

Beatrix Ueberheide
contact affiliation	NYU Langone Health
contact email	beatrix.ueberheide@nyulangone.org
lab head
Beatrix Ueberheide
contact affiliation	NYU School of Medicine
contact email	beatrixueberheide@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092907/