PXD045483-2

PXD045483 is an original dataset announced via ProteomeXchange.

Title	Aged gastrocnemius muscle of mice positively responds to a late onset adapted physical training
Description	A regular physical training is known to contribute to preserve muscle mass and strength, maintaining structure and function of neural and vascular compartments and preventing muscle insulin resistance and inflammation. However, physical activity is progressively reduced during aging causing mobility limitations and poor quality of life. Although physical exercise for rehabilitation purposes (e.g., after fractures or cardiovascular events) or simply aiming to counteract the development of sarcopenia is frequently advised by physicians, nevertheless few data are available on the targets and the global effects on the muscle organ of adapted exercise especially if started at old age. To contribute answering this question for medical translational purposes, the proteomic profile of the gastrocnemius muscle was analyzed in 24-month-old mice undergoing adapted physical training on a treadmill for 12 weeks or kept under a sedentary lifestyle condition. Proteomic data were implemented by morphological and morphometrical ultrastructural evaluations. Data demonstrate that muscles can respond to adapted physical training started at old age, positively modulating their morphology and the proteomic profile fostering protective and saving mechanisms either involving muscle cell components and pathways (i.e., mitochondrial processes, translation pathways and protein refolding, contractile apparatus) as well as the extracellular compartment. Therefore, this study provides important insights on the targets of adapted physical training, which can be regarded as suitable benchmarks for future in vivo studies further exploring the effects of this type of physical activity by functional/metabolic approaches.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:25:41.410.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesco Demetrio Lofaro
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-19 04:23:59	ID requested
1	2023-11-06 03:30:51	announced
⏵ 2	2023-11-14 08:25:41	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Ageing, skeletal muscle, Matrix, physical training, Electron microscopy, Proteomics

Federica Boraldi
contact affiliation	University of Modena and Reggio Emilia
contact email	federica.boraldi@unimore.it
lab head
Francesco Demetrio Lofaro
contact affiliation	University of Modena and Reggio Emilia
contact email	francescodemetrio.lofaro@unimore.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045483
     1. Label: PRIDE project
     2. Name: Aged gastrocnemius muscle of mice positively responds to a late onset adapted physical training