PXD045482 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proximal proteins of the type I interferon signaling cascade |
Description | Deciphering the intricate dynamic events governing type I interferon (IFN) signaling is critical to unravel key regulatory mechanisms in host antiviral defense. Here, we leveraged TurboID-based proximity labeling coupled with affinity purification-mass spectrometry to comprehensively map temporal changes to the proximal human proteomes of all seven canonical type I IFN signaling cascade members following IFN stimulation. This established a network of 108 proteins in close proximity to the core members IFNAR1, IFNAR2, JAK1, TYK2, STAT1, STAT2, and IRF9, and validated several known protein assemblies, while also revealing novel, transient associations between key signaling molecules. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-28 |
AnnouncementXML | Submission_2024-05-28_05:30:31.188.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Samira Schiefer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-19 03:28:02 | ID requested | |
⏵ 1 | 2024-05-28 05:30:32 | announced | |
Publication List
10.1038/s41467-024-48800-5; |
Schiefer S, Hale BG, Proximal protein landscapes of the type I interferon signaling cascade reveal negative regulation by PJA2. Nat Commun, 15(1):4484(2024) [pubmed] |
Keyword List
submitter keyword: IFN, TurboID, interferon, proximity labeling, JAK/STAT |
Contact List
Ben Hale |
contact affiliation | Institute of Medical Virology, University of Zurich, Switzerland |
contact email | hale.ben@virology.uzh.ch |
lab head | |
Samira Schiefer |
contact affiliation | Institute of Medical Virology, University of Zurich |
contact email | schiefer.samira@virology.uzh.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD045482 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045482
- Label: PRIDE project
- Name: Proximal proteins of the type I interferon signaling cascade