PXD045457 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | 15 cm Aurora Gen3 column benchmarking on an Exploris 480 |
Description | A comprehensive proteome map is essential to elucidate molecular pathways and protein functions. Although great improvements in sample preparation, instrumentation and data analysis already yield-ed impressive results, current studies suffer from a limited proteomic depth and dynamic range there-fore lacking low abundant or highly hydrophobic proteins. Here, we combine and benchmark advanced micro pillar array columns (µPAC) operated at nanoflow with Wide Window Acquisition (WWA) and the AI-based CHIMERYS search engine for data analysis to maximize chromatographic separation power, sensitivity and proteome coverage. Our data shows that µPAC columns clearly outperform classical packed bed columns boosting peptide IDs by up to 50% and protein IDs by up to 24%. Using the above-mentioned analysis platform, more than 10,000 proteins could be identified from a single 2 h gradient shotgun analysis for a triple proteome mix of human, yeast and E. coli digests. At high sample loads of 400 ng all three uPAC types yielded comparable number of protein identifications, whereas the 50cm neo column performed best when lower inputs of less than 200 ng were injected. This additional dataset comprises additional data generated with the Aurora Elite G3 column (150 mm x 75 µm, 1.7 µm, IonOpticks) for comparison to the aforementioned µPAC technology. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-13 |
AnnouncementXML | Submission_2024-02-13_04:20:54.307.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rupert Mayer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-18 05:31:25 | ID requested | |
⏵ 1 | 2024-02-13 04:20:55 | announced | |
Publication List
Matzinger M, Schm, ü, cker A, Yelagandula R, Stejskal K, Kr, š, š, á, kov, á G, Berger F, Mechtler K, Mayer RL, Micropillar arrays, wide window acquisition and AI-based data analysis improve comprehensiveness in multiple proteomic applications. Nat Commun, 15(1):1019(2024) [pubmed] |
10.1038/s41467-024-45391-z; |
Keyword List
submitter keyword: Vanquish Neo,Orbitrap Exploris 480, trap-and-elute |
Contact List
Karl Mechtler |
contact affiliation | Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria. |
contact email | karl.mechtler@imp.ac.at |
lab head | |
Rupert Mayer |
contact affiliation | Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC) |
contact email | rupert.mayer@imp.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045457
- Label: PRIDE project
- Name: 15 cm Aurora Gen3 column benchmarking on an Exploris 480