PXD045450 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of RSK3 interactome in human cells |
Description | Cellular senescence is characterized by a proliferation arrest accompanied by a senescence-associated secretory phenotype (SASP) in normal epithelial cells. During carcinogenesis cells lose the ability to undergo senescence, at least the associated proliferation arrest, in response to stresses such as oncogenes, or TGFβ, a SASP molecule able to mediate autocrine and paracrine senescence. A genetic screen in human mammary epithelial cells (HMECs) identified that the serine threonine kinase RSK3 reverts TGFβ-induced senescence. Interestingly, RSK3 expression decreases in response to TGFβ in a SMAD3-dependent manner, and its constitutive expression rescues SMAD3-induced premature senescence, indicating that decrease of RSK3 itself contributes to TGFβ-induced senescence. Mechanistically, RSK3 regulates senescence by inhibiting NF-κΒ pathway through the decrease in proteasome-mediated IκBα degradation. To better understand how RSK3 might interfere with IκBα degradation, we conducted immunoprecipitation experiments to purify the RSK3-Flag protein and its partners. Mass spectrometry-based proteomics was then performed to identify potential partners. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-27 |
AnnouncementXML | Submission_2023-11-27_02:49:50.670.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD045450 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Yohann Couté |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-18 04:43:27 | ID requested | |
⏵ 1 | 2023-11-27 02:49:51 | announced | |
Publication List
Keyword List
submitter keyword: RSK3,Senescence, TGFβ, Epithelial-mesenchymal transition, immunoprecipitation, nanoLC-MS/MS |
Contact List
Yohann Couté |
contact affiliation | EDyP |
contact email | yohann.coute@cea.fr |
lab head | |
Yohann Couté |
contact affiliation | EDyP |
contact email | yohann.coute@cea.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD045450 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045450
- Label: PRIDE project
- Name: Characterization of RSK3 interactome in human cells