PXD045429-1

PXD045429 is an original dataset announced via ProteomeXchange.

Title	Tumor mitochondrial DNA and antigen-loaded nanoparticles encapsulated with dendritic cell derived microvesicles elicits long lasting anti-tumor immune responses by activating the cGAS-STING pathway
Description	Scarcity of dendritic cells (DCs) impact lung and pancreatic tumor immune status despite limited clinical benefits from autologous tumor lysate-pulsed DC vaccination. To tackle this issue, we developed a DC-based immunotherapy utilizing cationic nanoparticles (cNPs) loaded with tumor or organoid lysate encapsulated within DC-derived microvesicles (cNPcancer cell@MVDC). Remarkably, cNPcancer cell@MVDC treatment converted immune cold tumors into a hot microenvironment, leading to increased migratory DC population, reduced tumor growth and improved survival in orthotopic animal models compared to mature DC treatment. In vivo tracking experiments demonstrated superior accumulation of cNPcancer cell@MVDC in tumors and draining lymph nodes (dLNs) compared to mature DCs, promoting DC migration to dLNs and activating CD8+ T cells. Clinically, the accurate prediction of tumor immune status, immunotherapy response and patient prognosis relies on migratory DC infiltration rather than CD8+ T cells. Mechanistically, tumor lysate pulsed-cNPs enriched mitochondrial DNA, which exhibited a stronger binding affinity with cGAS compared to nuclear DNA, resulting in enhanced cGAS-STING-mediated DC activation. This immunotherapy elicits durable anti-tumor immune responses, even in immune-deserted tumor environments.
HostingRepository	iProX
AnnounceDate	2023-09-16
AnnouncementXML	Submission_2024-05-21_18:43:23.392.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xue Jiang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-17 20:55:59	ID requested
⏵ 1	2024-05-21 18:43:23	announced

Dataset with its publication pending

submitter keyword: Pancreatic ductal adenocarcinomas, Dendritic cell, Microvesicles, cGAS, mitochondrial DNA

Pingpui Wong
contact affiliation	Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University
contact email	huangbp3@mail.sysu.edu.cn
lab head
Xue Jiang
contact affiliation	Sun Yat-sen Memorial Hospital, Sun Yat-sen University
contact email	915143522@qq.com
dataset submitter

iProX dataset URI