PXD045419-1

PXD045419 is an original dataset announced via ProteomeXchange.

Title	Protein palmitoylation and sphingolipid metabolism cooperatively 1 control regulated exocytosis in cytotoxic lymphocytes
Description	The cytotoxic activity of lymphocytes is particularly dependent on the regulated and polarised delivery of lytic granules to infected or malignant cells. Although genetic and mechanistic studies have identified a number of factors that regulate exocytosis in cytotoxic lymphocytes, a systematic mapping of the relevant factors and their relationships is lacking. Here, using a genome-scale CRISPR knockout screen in human natural killer cells, we characterise a complex genetic network regulating cytotoxic granule exocytosis, with lipid metabolism and protein lipidation among the most prominent pathways. By combining global protein lipidation and membrane lipid composition studies, we further uncover the critical role of ZDHHC17 in SNAP23 palmitoylation and targeting of palmitoylated SNAP23 to the membrane for cytotoxic granule fusion and release. Using ODYA-17 labelling of Ctrl and sgZDHHC17 NK-92 cells followed by pulldown and proteomic analysis, we investigated the palmitoylome of the NK-92 model cell line. By comparing the abundance of ODYA-17 labelled proteins in the lysates of Ctrl and sgZDHHC17 NK-92 cells, we defined the molecules that depend on the activity of the palmitoyl transferase ZDHHC17.
HostingRepository	PRIDE
AnnounceDate	2025-09-18
AnnouncementXML	Submission_2025-09-18_08:59:34.055.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Rukavina
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-15 10:17:51	ID requested
⏵ 1	2025-09-18 08:59:34	announced

Dataset with its publication pending

submitter keyword: exocytosis,NK cells, palmitoylation

Kaan Boztug
contact affiliation	1. St. Anna Children’s Cancer Research Institute (CCRI), Vienna, Austria 2. CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria 3. Ludwig Boltzmann Institute for Rare and Undiagnosed Diseases, Vienna, Austria 4. Medical University of Vienna, Department of Pediatrics and Adolescent Medicine, Vienna, Austria 5. St. Anna Children’s Hospital, Medical University of Vienna, Department of Pediatrics and Adolescent Medicine, Vienna, Austria
contact email	kaan.boztug@ccri.at
lab head
Andrea Rukavina
contact affiliation	The CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
contact email	arukavina@cemm.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD045419

PRIDE project URI

• PRIDE
  1. PXD045419
     1. Label: PRIDE project
     2. Name: Protein palmitoylation and sphingolipid metabolism cooperatively 1 control regulated exocytosis in cytotoxic lymphocytes