PXD045411-2

PXD045411 is an original dataset announced via ProteomeXchange.

Title	Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling
Description	Accurate chromosome segregation is coordinated by the spindle assembly checkpoint (SAC) through its effector the mitotic checkpoint complex (MCC), to inhibit the anaphase-promoting complex or cyclosome (APC/C). Cdc20 is an essential mitotic regulator since it promotes mitotic exit through activating the APC/C and monitors kinetochore-microtubule attachment through activating the SAC. The proper functioning of Cdc20 requires multiple interactions with APC/C and MCC subunits. To functionally assess each of these interactions within cells requires efficient depletion of endogenous Cdc20, which is highly difficult to achieve by RNAi. Here we generated Cdc20 RNAi sensitive cell lines by CRISPR/Cas9 which display a penetrant metaphase arrest phenotype by a single RNAi treatment. In this null background, we accurately measured the contribution of each known motif of Cdc20 on APC/C and SAC activation. The CRY box, a previously identified degron was found to be critical for the SAC by promoting theMCC formation and stabilizing the interaction between the MCC and APC/C. These data reveal additionalregulatory components within the SAC and establish a novel method to interrogate Cdc20 function.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:16:51.409.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuqing Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-15 03:20:31	ID requested
1	2023-12-05 17:33:53	announced
⏵ 2	2024-10-22 06:16:51	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Cdc20;CRY box;CRISPR Cas9;mitosis

Gang Zhang
contact affiliation	Cancer Institute, The Affiliated Hospital of Qingdao University, Qingdao University,Qingdao, China
contact email	zhanggang_sma@qdu.edu.cn
lab head
Yuqing Zhang
contact affiliation	Qingdao University
contact email	yuqingzh97@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045411
     1. Label: PRIDE project
     2. Name: Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling