PXD045379-1

PXD045379 is an original dataset announced via ProteomeXchange.

Title	A conserved cell-pole determinant organizes proper polar flagellum formation
Description	The coordination of cell cycle progression and flagellar synthesis is a complex process in motile bacteria. In γ-proteobacteria, the localization of the flagellum to the cell pole is mediated by the SRP-type GTPase FlhF. However, the mechanism of action of FlhF, and its relationship with the cell pole landmark protein HubP remain unclear. In this study, we discovered a novel protein called FipA that is required for normal FlhF activity and function in polar flagellar synthesis. We demonstrated that membrane-localized FipA interacts with FlhF and is required for normal flagellar synthesis in Vibrio parahaemolyticus, Pseudomonas putida, and Shewanella putrefaciens, and it does so independently of the polar localization mediated by HubP. FipA exhibits a dynamic localization pattern and is present at the designated pole before flagellar synthesis begins, suggesting its role in licensing flagellar formation. This discovery provides insight into a new pathway for regulating flagellum synthesis and coordinating cellular organization in bacteria that rely on polar flagellation and FlhF-dependent localization.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_11:39:28.156.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Vibrio parahaemolyticus BB22OP; NCBI TaxID: 1211705;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-14 00:56:39	ID requested
⏵ 1	2025-05-06 11:39:29	announced

Arroyo-P, é, rez EE, Hook JC, Alvarado A, Wimmi S, Glatter T, Thormann K, Ringgaard S, A conserved cell-pole determinant organizes proper polar flagellum formation. Elife, 13():(2024) [pubmed]

10.7554/elife.93004;

submitter keyword: flagellar synthesis, FlhF, FlhG, FimV, cell pole, HubP

Timo Glatter
contact affiliation	Max Planck Insitute for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany
contact email	timo.glatter@mpi-marburg.mpg.dee
lab head
Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Marburg
contact email	timo.glatter@mpi-marburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD045379

PRIDE project URI

• PRIDE
  1. PXD045379
     1. Label: PRIDE project
     2. Name: A conserved cell-pole determinant organizes proper polar flagellum formation