PXD045334-1
PXD045334 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of interacting proteins with the NP protein of the non-oncolytic H-NP366-489I strain and the non-oncolytic Herts/33 strain by mass spectrometry |
Description | Newcastle disease virus (NDV) is one of the most significant threats to the poultry industry, and it also exhibits oncolytic properties. In our research, we have identified a non-oncolytic strain, the genotype VII NDV strain I4. We have found that the lack of oncolytic characteristics in this strain is attributed to amino acids 366-489 in the NP protein. To delve into the mechanism of NP-mediated NDV oncolysis, we initiated further investigations using the highly oncolytic Herts/33 strain's NP and a recombinant NP protein with amino acids 366-489 from the I4 strain substituted in (H-NP366-489I). These proteins represent the interacting partners in the proteomics analysis of NP proteins from both viral strains. After infecting HeLa cells with each of these strains, immunoprecipitation was performed using antibodies targeting the NP protein. Since the H-NP366-489I virus produces fewer proteins during replication, we additionally overexpressed the H-NP366-489I and Herts/33 NP proteins separately for immunoprecipitation experiments. |
HostingRepository | iProX |
AnnounceDate | 2023-09-12 |
AnnouncementXML | Submission_2023-09-12_19:14:18.286.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tianxing Liao |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-09-12 19:13:43 | ID requested | |
⏵ 1 | 2023-09-12 19:14:18 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mass spectrometry, Newcastle disease virus, NP protein, oncolytic virus |
Contact List
Xiufan Liu | |
---|---|
contact affiliation | Yangzhou University |
contact email | xfliu@yzu.edu.cn |
lab head | |
Tianxing Liao | |
contact affiliation | Yangzhou University |
contact email | 2693040819@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |