PXD045239-2

PXD045239 is an original dataset announced via ProteomeXchange.

Title	Adaptive design of mRNA-loaded extracellular vesicles for targeted immunotherapy of cancer
Description	Extracellular vesicles (EVs) have emerged as promising delivery vehicles for RNA-based therapeutics because of their advantages over other mRNA delivery systems, including their excellent biosafety and biocompatibility, stability against degradation, and the ability to cross physiological barriers such as the blood-brain barrier. In this study, we report a nanosecond electroporation (nsEP) system with a microfluidic configuration. Applying millisecond and nanosecond pulses separately shifted the main impact of electroporation from the cell membrane to the membrane structure of cellular organelles. After being treated by a nsEP system, mouse embryonic fibroblasts (MEFs) demonstrated the capability to generate large quantities of small extracellular vesicles (sEVs). To gain a better understanding of the biological mechanisms underlying this nsEP-triggered sEV release, we conducted a proteomics analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:02:57.045.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shiyan Dong
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-09 02:20:54	ID requested
1	2023-09-09 20:09:23	announced
⏵ 2	2024-10-22 06:02:57	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: glioblastoma, nanosecond pulse electroporation,extracellular vesicle

Wen Jiang
contact affiliation	Associate Professor
contact email	wjiang4@mdanderson.org
lab head
Shiyan Dong
contact affiliation	Postdoctoral fellow
contact email	dongshiyan06@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD045239

PRIDE project URI

• PRIDE
  1. PXD045239
     1. Label: PRIDE project
     2. Name: Adaptive design of mRNA-loaded extracellular vesicles for targeted immunotherapy of cancer