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PXD045214-1

PXD045214 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe PIWI-interacting protein Gtsf1 controls the selective degradation of small RNAs in Paramecium
DescriptionCiliates undergo developmentally programmed genome elimination, in which small RNAs direct the removal of target DNA segments, including transposable elements. At each sexual generation, the development of the macronucleus (MAC) requires massive and reproducible elimination of a large proportion of the germline micronuclear (MIC) genome, leading to a highly streamlined somatic MAC genome. 25-nt long scnRNAs are produced from the entire germline MIC genome during meiosis, and this initial complex small RNA population is then transported to the maternal MAC, where selection of scnRNAs corresponding to germline (MIC)-specific sequences is thought to take place. Selected scnRNAs, loaded onto the PIWI protein Ptiwi09, guide the deposition of histone H3 post-translational modifications (H3K9me3 and H3K27me3) onto transposable elements in the developing macronucleus, ultimately triggering their specific elimination. How germline-specific MIC scnRNAs are selected remains to be determined. Here, we provide important mechanistic insights into the scnRNA selection pathway by identifying a Paramecium homolog of Gametocyte specific factor 1 (Gtsf1) as essential for the selective degradation of scnRNAs corresponding to retained somatic MAC sequences. Consistently, we also show that Gstf1 is exclusively localized in the maternal macronucleus, where scnRNA selection is presumed to occur, and associates with the scnRNA-binding protein Ptiwi09. Furthermore, Gtsf1 is necessary for DNA elimination and correct H3K9me3 and H3K27me3 localization in the new developing macronucleus, demonstrating that the scnRNA selection process is important for genome elimination. We propose that Gtsf1 is required for the coordinated degradation of Ptiwi09-scnRNA complexes that pair with nascent RNA transcribed from the maternal MAC genome, similarly to the mechanism suggested for microRNA target-directed degradation in metazoans.
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_01:47:45.806.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuillaume CHEVREUX
SpeciesList scientific name: Paramecium tetraurelia; NCBI TaxID: 5888;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro 2
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-08 02:13:24ID requested
12024-10-17 01:47:46announced
Publication List
10.1101/2023.09.19.558372;
Keyword List
submitter keyword: paramecium, transposable elements, scnRNAs selection,Gtsf1
Contact List
Sandra Duharcourt
contact affiliationUniversité Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France
contact emailsandra.duharcourt@ijm.fr
lab head
Guillaume CHEVREUX
contact affiliationInstitut Jacques Monod, CNRS UMR7592
contact emailguillaume.chevreux@ijm.fr
dataset submitter
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