PXD045129-1

PXD045129 is an original dataset announced via ProteomeXchange.

Title	An obligate aerobe adapts to hypoxia by hybridising fermentation with carbon storage
Description	In soil ecosystems, obligately aerobic bacteria survive oxygen deprivation (hypoxia) by entering non-replicative persistent states. Little is known about how these bacteria rewire their metabolism to stay viable in these states. One obligate aerobe, Mycobacterium smegmatis, maintains redox homeostasis during hypoxia by mediating fermentative hydrogen production. However, the fate of organic carbon during fermentation, and the associated remodeling of carbon metabolism, is unresolved. Here we systematically profiled the metabolism of M. smegmatis during aerobic growth, hypoxic persistence, and the transition between these states. Using differential isotope labelling, and paired metabolomics and proteomics, we observed rerouting of central carbon metabolism through the pentose phosphate pathway and Entner-Doudoroff pathway (during hypoxia. We show that M. smegmatis excretes high levels of hydrogen concurrently with upregulating triacylglyceride synthases and accumulating glycerides as carbon stores. Using electron cryotomography (cryo-ET), we observed the presence of large spheroid structures consistent with the appearance of lipid droplets. Thus, in contrast to obligately and facultative anaerobic fermentative bacteria, M. smegmatis stores rather than excretes organic carbon during hypoxia. This novel hybrid metabolism likely provides a competitive advantage in resource-variable environments by allowing M. smegmatis to simultaneously dispose excess reductant during hypoxia and maintain carbon stores to rapidly resume growth upon reoxygenation.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-08_00:10:01.671.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Han Lee
SpeciesList	scientific name: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155); NCBI TaxID: NEWT:246196;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-05 21:27:22	ID requested
⏵ 1	2026-06-08 00:10:02	announced

10.1073/PNAS.2606389123;

submitter keyword: oxygen, dormancy, persistence, triacylglyceride,Mycobacteria, hypoxia, fermentation, hydrogenase, triacylglycerol

Chris Greening
contact affiliation	Department of Microbiology, Monash University, Australia
contact email	chris.greening@monash.edu
lab head
Han Lee
contact affiliation	Monash Proteomics & Metabolomics Facility
contact email	han.lee@monash.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045129
     1. Label: PRIDE project
     2. Name: An obligate aerobe adapts to hypoxia by hybridising fermentation with carbon storage