PXD045109-1

PXD045109 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Protein N-Terminomics Reveals Major Proteases in Regulating Beige Adipocyte Differentiation
Description	We have developed an analytical platform to measure the temporal profiles of the proteome and the protein N-terminome during beige adipocyte differentiation. Our recently developed N-terminomics approach was applied to isolate protein N-terminal peptides with up to 97% purity. In total, we identified 7,171 unique N- terminal peptides, of which 3,043 were from canonical proteins (CanNt peptides) and 4,129 were from proteins with neo-N-termini (NeoNt peptides). We found that 44% of proteins showed different temporal profiles between the global proteome and protein N-terminome. Our results indicate that proteolysis plays a major role in regulating beige adipocyte differentiation. Knockdown of Pmpbc, Plg or Cstd in preadipocytes impeded thermogenesis by decreasing Cidea, Pgc1a and Ucp1 expression and elevating adipogenic markers such as Fasn and Fabp4, thus promoting the beige-to-white adipocyte transition. Our approach reveals that proteases and the generated proteolysis products play major roles in beige adipocyte differentiation.
HostingRepository	jPOST
AnnounceDate	2025-09-05
AnnouncementXML	Submission_2025-09-04_08:00:05.634.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hiroshi Nishida
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; TMT; Acetyl; alpha-amino acetylated residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-05 07:31:53	ID requested
⏵ 1	2025-09-04 08:00:06	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: N-terminomics, adipocyte differentiation, proteomics, temporal profiling, proteolysis, protease

submitter keyword: N-terminomics, adipocyte differentiation, proteomics, temporal profiling, proteolysis, protease

Contact List

Yasushi Ishihama
lab head
Hiroshi Nishida
contact affiliation	Kyoto university
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002309/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002309/