PXD045075 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The role of inverse autotransporter FdeC in Avian Pathogenic Escherichia coli adhesion |
Description | Avian Pathogenic Escherichia coli (APEC) poses a significant threat to the global poultry industry and has also zoonotic potential, meaning it could infect humans. One critical factor in APEC infection is its ability to adhere to host tissues, a process mediated by a protein called FdeC among others. This protein is present in pathogenic E. coli strains but is often nonfunctional in nonpathogenic ones. Our study focused on understanding the role of FdeC in APEC's ability to bind to chicken intestinal cells. Through an extensive screen of 2000 transposon mutants from the APEC IMT5155 strain, we discovered that a specific mutation in the fdeC gene led to enhanced adhesion. However, a direct deletion of the fdeC gene did not produce the same effect under standard conditions, which led us to investigate the environmental factors that might regulate FdeC expression. We found that FdeC's expression is highly dependent on the environmental conditions such as temperature and pH. Using optimized conditions for FdeC expression, we observed significantly higher adhesion and motility levels in the fdeC-deleted IMT5155 strain compared to the wild type. To understand the mechanisms behind this, we employed mass spectrometry and discovered that the absence of FdeC led to changes in ion transport and downregulated a protein called YbjN, which usually inhibits bacterial movement. Collectively, our findings suggest that FdeC has host-dependent expression and contributes to enhancement of bacterial fitness by modulating motility during colonization. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-16 |
AnnouncementXML | Submission_2024-06-15_23:15:49.113.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fernando Garcia |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-04 03:59:26 | ID requested | |
⏵ 1 | 2024-06-15 23:15:50 | announced | |
Publication List
10.1186/s13567-024-01327-5; |
Aleksandrowicz A, Kj, æ, rup RB, Grzymaj, ł, o K, Martinez FG, Mu, ñ, oz J, Borowska D, Sives S, Vervelde L, Dalgaard TS, Kingsley RA, Kolenda R, FdeC expression regulates motility and adhesion of the avian pathogenic Escherichia coli strain IMT5155. Vet Res, 55(1):70(2024) [pubmed] |
Keyword List
ProteomeXchange project tag: EPIC-XS |
submitter keyword: Avian Patogenic Escheirichia. FdeC |
Contact List
Fernando Garcia Martinez |
contact affiliation | Fernando Garcia Martinez Ph.D. Proteomics & MS Specialist Proteomics Unit, Spanish National Cancer Research Centre (CNIO) Melchor Fernández Almagro 3. E28029 MADRID, SPAIN. Phone +34 912246900 ext 3112 |
contact email | fgarcia@cnio.es |
lab head | |
Fernando Garcia |
contact affiliation | Fernando García Martínez, Ph.D. Proteomics Unit, Spanish National cancer Research Center, CNIO, MADRID, SPAIN |
contact email | fgarcia@cnio.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045075
- Label: PRIDE project
- Name: The role of inverse autotransporter FdeC in Avian Pathogenic Escherichia coli adhesion