PXD045074 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The tumour-derived extracellular vesicle proteome varies by endometrial cancer histology and is confounded by an obesogenic environment |
| Description | Endometrial cancer is the most prevalent gynaecological cancer globally. Its association with obesity and metabolic diseases is a key aetiology, increasingly among younger females. Early diagnosis and improved treatment decisions are crucial for these individuals which could be fulfilled by the discovery of new biomarkers. We took a new approach to extracellular vesicle (EV) biomarker discovery - profiling the proteome of enriched EVs isolated directly from frozen biobanked endometrial cancers. Nine tissue pools, each generating collagenase-digested tissue and matched small EVs, were analysed using label-free proteomics. Three clinical subgroups: Endometrioid low BMI (body mass index), Endometrioid high BMI, and Serous, irrespective of BMI were compared to identify shared secreted proteins, proteins associated with histological subtype, and proteins related to BMI. EVs were enriched for common EV markers and large secreted proteins. Cell lysates were enriched in mitochondrial and blood proteins. EV protein profiles were most different between the high BMI subgroup and the others, highlighting a significant influence of co-morbidities on the intra-tumoural EV secretome. Proteins that were differentially abundant between subgroups in tissues, were strikingly not also differential in the matched EVs. This work has identified secreted proteins implicated in the complex pathophysiology of endometrial cancer and pinpointed candidate biomarkers for diagnosis. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-09-24 |
| AnnouncementXML | Submission_2024-09-23_18:13:03.420.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD045074 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | George, G GUO |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-09-04 03:28:08 | ID requested | |
| ⏵ 1 | 2024-09-23 18:13:04 | announced | |
Publication List
| 10.1002/pmic.202300055; |
| Artuyants A, Guo G, Flinterman M, Middleditch M, Jacob B, Lee K, Vella L, Su H, Wilson M, Eva L, Shelling AN, Blenkiron C, The tumour-derived extracellular vesicle proteome varies by endometrial cancer histology and is confounded by an obesogenic environment. Proteomics, 24(11):e2300055(2024) [pubmed] |
| 10.6019/PXD045074; |
Keyword List
| submitter keyword: SWATH,biomarkers, extracellular vesicles, endometrial cancer, secretome |
Contact List
| Cherie Blenkiron |
|---|
| contact affiliation | Molecular Medicine and Pathology, The University of Auckland, NZ |
| contact email | c.blenkiron@auckland.ac.nz |
| lab head | |
| George, G GUO |
|---|
| contact affiliation | The University of Auckland |
| contact email | guoguodigital@msn.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD045074 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045074
- Label: PRIDE project
- Name: The tumour-derived extracellular vesicle proteome varies by endometrial cancer histology and is confounded by an obesogenic environment
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