PXD045072-1

PXD045072 is an original dataset announced via ProteomeXchange.

Title	Helicobacter pylori induces a novel form of innate immune memory via accumulation of NF-кB proteins
Description	Helicobacter pylori is a widespread Gram-negative pathogen involved in a variety of gastrointestinal diseases, including gastritis, ulceration, mucosa-associated lymphoid tissue (MALT) lymphoma and gastric cancer. Immune responses aimed at eradication of H. pylori often prove futile, and paradoxically play a crucial role in the degeneration of epithelial integrity and disease progression. We have previously shown that H. pylori infection of primary human monocytes increases their potential to respond to subsequent bacterial stimuli – a process that may be involved in the generation of exaggerated, yet ineffective immune responses directed against the pathogen. In this study, we show that H. pylori-induced monocyte priming is not a common feature of Gram-negative bacteria, as Acinetobacter lwoffii induces tolerance to subsequent Escherichia coli lipopolysaccharide (LPS) challenge. Although the increased reactivity of H. pylori-infected monocytes seems to be specific to H. pylori, it appears to be independent of its virulence factors Cag pathogenicity island (CagPAI), cytotoxin associated gene A (CagA), vacuolating toxin A (VacA) and g-glutamyl transferase (g-GT). Utilizing whole-cell proteomics complemented with biochemical signaling studies, we show that H. pylori infection of monocytes induces a unique proteomic signature compared to other pro-inflammatory priming stimuli, namely LPS and the pathobiont A. lwoffii. Contrary to these tolerance- inducing stimuli, H. pylori priming leads to accumulation of NF-кB proteins, including p65/RelA, and thus to the acquisition of a monocyte phenotype more responsive to subsequent LPS challenge. The plasticity of pro-inflammatory responses based on abundance and availability of intracellular signaling molecules may be a heretofore underappreciated form of regulating innate immune memory as well as a novel facet of the pathobiology induced by H. pylori
HostingRepository	PRIDE
AnnounceDate	2023-11-10
AnnouncementXML	Submission_2023-11-10_00:48:32.224.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	VeronikaSchäpertöns
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-04 02:08:46	ID requested
⏵ 1	2023-11-10 00:48:32	announced
2	2023-11-14 08:26:34	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:13:54	announced	2024-10-22: Updated project metadata.

submitter keyword: TMT labelled

ChristofRegl
contact affiliation	Bioanalytical Research Labs, Department of Biosciences & Medical Biology, Universität Salzburg, Austria
contact email	christof.regl@plus.ac.at
lab head
VeronikaSchäpertöns
contact affiliation	PLUS University of Salzburg
contact email	veronika.schaepertoens@protonmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045072
     1. Label: PRIDE project
     2. Name: Helicobacter pylori induces a novel form of innate immune memory via accumulation of NF-кB proteins