PXD045057-2
PXD045057 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic characterization of extracellular matrix (ECM) produced by human fibroblast activation protein (FAP)-positive pericyte-like cells and glioma cells |
Description | Fibroblast activation protein (FAP) is upregulated in most cancers and represents an emerging theranostic target in oncology. In glioblastoma (GBM), FAP is predominantly expressed in mesenchymal pericyte-like cells, which contribute to GBM progression by promoting angiogenesis and glioma cell proliferation by soluble mediators. In this work, we show another possible mechanism by which these cells contribute to gliomagenesis. We observed that FAP+ pericyte-like cells residing in the perivascular niche in GBM are associated with elevated levels of the fibrillar extracellular matrix (ECM) proteins collagen I and fibronectin. Using sequential window acquisition of all theoretical mass spectra (SWATH-MS) analysis, we confirmed that the highest levels of collagen I and fibronectin are produced by FAP+ pericyte-like cells. In addition, we observed different expression pattern of basal membrane proteins between FAP+ pericyte-like cells and glioma cells. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2024-05-07 |
AnnouncementXML | Submission_2024-05-07_11:06:43.169.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jakub Cervenka |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | Acetyl; Carbamidomethyl; Oxidation |
Instrument | TripleTOF 5600+ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-09-03 15:20:54 | ID requested | |
1 | 2024-05-02 12:48:42 | announced | |
⏵ 2 | 2024-05-07 11:06:44 | announced | : Added PubMed Id |
Publication List
Vymola P, Garcia-Borja E, Cervenka J, Balaziova E, Vymolova B, Veprkova J, Vodicka P, Skalnikova H, Tomas R, Netuka D, Busek P, Sedo A, Fibrillar extracellular matrix produced by pericyte-like cells facilitates glioma cell dissemination. Brain Pathol, ():e13265(2024) [pubmed] |
Keyword List
submitter keyword: Glioblastoma, Collagen I, Fibronectin, Fibroblast activation protein, Extracellular matrix, SWATH-MS |
Contact List
Petr Vodička | |
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contact affiliation | Laboratory of Applied Proteome Analyses, Research Center PIGMOD, Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, Czech Republic |
contact email | vodicka@iapg.cas.cz |
lab head | |
Jakub Cervenka | |
contact affiliation | Laboratory of Applied Proteome Analyses, Research Center PIGMOD, Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, Czech Republic |
contact email | cervenka@iapg.cas.cz |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |