PXD045049-1

PXD045049 is an original dataset announced via ProteomeXchange.

Title	Simultaneous xylose and glucose utilization by an E. coli crp mutant
Description	As advances are made toward the industrial feasibility of mass-producing biofuels and commodity chemicals with sugar-fermenting microbes, high feedstock costs continue to inhibit commercial application. Hydrolyzed lignocellulosic biomass represents an ideal feedstock for these purposes as it is cheap and prevalent. However, many microbes, including Escherichia coli, struggle to efficiently utilize this mixture of hexose and pentose sugars due to the regulation by the carbon catabolite repression (CCR) system. CCR causes a sequential utilization of sugars, rather than simultaneous utilization, resulting in reduced carbon yield and complex process implications in fed-batch fermentation. A mutation in the gene encoding the cyclic AMP receptor protein, Crp*, has been shown to disable CCR and improve co-utilization of mixed sugar substrates. Here, we present the strain construction and characterization of a site specific crp* chromosomal mutant in E. coli BL21 starTM (DE3). The crp* mutant strain demonstrates simultaneous consumption of glucose and xylose, suggesting a deregulated CCR system. The proteomic analysis further showed that cells link xylose consumption to energy production through the de novo nucleotide synthesis pathway, explaining the relatively slower growth of the crp* mutant strain. This highly characterized strain can be particularly beneficial for chemical production by simultaneously utilizing both C5 and C6 substrates from lignocellulosic biomass.
HostingRepository	MassIVE
AnnounceDate	2023-12-21
AnnouncementXML	Submission_2023-12-21_07:13:42.408.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	XIN WANG
SpeciesList	scientific name: Escherichia coli; common name: E. coli; NCBI TaxID: 562;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-02 12:20:29	ID requested
⏵ 1	2023-12-21 07:13:42	announced

no publication

submitter keyword: CRP, catabolite repression, lignocellulose

Xin Wang
contact affiliation	University of Florida
contact email	wangx3@ufl.edu
lab head
XIN WANG
contact affiliation	University of Florida
contact email	wangx3@ufl.edu
dataset submitter

MassIVE dataset URI

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