PXD045003 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global proteome of PTH1R transfected U2OS cells |
Description | Brachydactyly type E (BDE), shortened metacarpals, metatarsals, cone-shaped epiphyses, and short stature, is an autosomal-dominant condition that commonly occurs as a sole phenotype. Parathyroid hormone-like protein (PTHrP) has been shown to be responsible in all forms to date, either directly or indirectly. We used linkage and then whole genome sequencing in a small pedigree, to elucidate BDE and identified a truncateddisintegrin-and-metalloproteinase-19 (ADAM19) allele in all affected family members, but not in nonaffected persons. Since we had shown earlier that the extracellular domain of the parathyroid hormone receptor (PTHR1) is subject to an unidentified metalloproteinase cleavage, we tested the hypothesis that ADAM19 is a sheddase for PTHR1. We transfected U2OS cells with ADAM19 (Control), PTH1R (disease-model), both PTH1R plus ADAM19 (healthy), and no transfection at all as another control. We did global proteome on these cell lines and compared them using a student t test. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-15 |
AnnouncementXML | Submission_2024-02-15_07:05:37.717.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Trendelina Rrustemi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-31 07:11:36 | ID requested | |
⏵ 1 | 2024-02-15 07:05:38 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: ADAM19, Brachydactyly Type E,PTH1R, Global Proteome |
Contact List
Matthias Selbach |
contact affiliation | Max Delbrueck Center for Molecular Medicine |
contact email | matthias.selbach@mdc-berlin.de |
lab head | |
Trendelina Rrustemi |
contact affiliation | Max-Delbrück Center for Molecular Medicine |
contact email | trendelina.rrustemi@mdc-berlin.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD045003 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045003
- Label: PRIDE project
- Name: Global proteome of PTH1R transfected U2OS cells