PXD044992-1

PXD044992 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Acetylomics on rhein treated NLRP3 activated J774A.1 macrophages
Description	The aim of this project is to observe the effect of 5 μM rhein treatment on the acetylation level of NLRP3 activated macrophages. We primed J774A.1 cells with 100ng/mL LPS for 12 hours followed by 5μM rhein treatment for 30 minutes. Finally, the cells were stimulated with 4 mM ATP for 30 min to trigger the inflammasome assembly. Cell lysates were collected after ATP stimulation by cell scrapers, and acetylomics were performed as described below.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:44:34.058.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruonan Zhou
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	timsTOF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-31 02:16:17	ID requested
⏵ 1	2024-10-17 02:44:34	announced

Publication List

10.1038/s42003-024-06693-6;
Zhou RN, Zhu ZW, Xu PY, Shen LX, Wang Z, Xue YY, Xiang YY, Cao Y, Yu XZ, Zhao J, Jin Y, Yan J, Yang Q, Fang PH, Shang WB, Rhein targets macrophage SIRT2 to promote adipose tissue thermogenesis in obesity in mice. Commun Biol, 7(1):1003(2024) [pubmed]

10.1038/s42003-024-06693-6;

Zhou RN, Zhu ZW, Xu PY, Shen LX, Wang Z, Xue YY, Xiang YY, Cao Y, Yu XZ, Zhao J, Jin Y, Yan J, Yang Q, Fang PH, Shang WB, Rhein targets macrophage SIRT2 to promote adipose tissue thermogenesis in obesity in mice. Commun Biol, 7(1):1003(2024) [pubmed]

Keyword List

submitter keyword: Rhein, macrophage, NLRP3 inflammasome

submitter keyword: Rhein, macrophage, NLRP3 inflammasome

Contact List

Wenbin Shang
contact affiliation	Key Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine
contact email	wbshang@hotmail.com
lab head
Ruonan Zhou
contact affiliation	Nanjing University of Chinese Medicine
contact email	Ruonan.Zhou@njucm.edu.cn
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD044992
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD044992

PRIDE project URI

Repository Record List

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