PXD044980-1

PXD044980 is an original dataset announced via ProteomeXchange.

Title	Paternal under and over nutrition modify semen composition and preimplantation embryo developmental kinetics in mice
Description	Poor parental health can influence the development and eventual health outcomes of their offspring. Many studies have detailed the maternal role yet a father’s health requires further examination as his poor nutritional status has also been shown to have negative consequences on fetal development and impact the long-term health of his offspring. The present study examined how preimplantation embryo development was altered by sub-optimal paternal diet, with specific focus on sperm- and seminal plasma-mediated mechanisms. To address this, male mice were fed a diet to model either under (low protein diet (LPD)) or over (high fat/sugar ‘Western’ diet (WD)) nutrition, LPD or WD supplemented with methyl-donors or a control diet (CD) before mating. Embryo development was assessed using in vitro time-lapse imaging of preimplantation embryos which revealed a significant increase in embryo development rates in all experimental groups when compared to CD embryos. Further analysis of the semen revealed a significant number of differentially expressed seminal plasma proteins in all groups (LPD: 13, MD-LPD: 27, WD: 24, MD-WD: 19) when compared to control. This study highlights a role for paternal nutritional status influencing embryonic development and the maternal reproductive tract via changes to his metabolic and reproductive health. These findings demonstrate potential direct (sperm-mediated) and indirect (seminal plasma-mediated) pathways in which a father's poor diet could shape the long-term health of his offspring
HostingRepository	PRIDE
AnnounceDate	2024-07-25
AnnouncementXML	Submission_2024-07-25_09:43:39.504.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Boocock
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-30 16:03:20	ID requested
⏵ 1	2024-07-25 09:43:39	announced

Dataset with its publication pending

submitter keyword: nutrition,mouse, proteomics, SWATH, seminal plasma

Dr David Boocock
contact affiliation	John van Geest Cancer Research Centre, Nottingham Trent University
contact email	david.boocock@ntu.ac.uk
lab head
David Boocock
contact affiliation	Nottingham Trent University
contact email	david.boocock@ntu.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044980
     1. Label: PRIDE project
     2. Name: Paternal under and over nutrition modify semen composition and preimplantation embryo developmental kinetics in mice