PXD044948-2

PXD044948 is an original dataset announced via ProteomeXchange.

Title	A Cell-Permeable Photosensitizer for Selective Proximity Labeling and Crosslinking of Aggregated Proteome
Description	Intracellular proteome aggregation is a ubiquitous disease hallmark with its composition associated with pathogenicity. Herein, we report on a cell-permeable photosensitizer (P8, Rose Bengal derivative) for selective photo-induced proximity labeling, crosslinking and identification of cellular aggregated proteome. Rose Bengal is identified out of common photosensitizer scaffolds for its unique intrinsic binding affinity to various protein aggregates driven by the hydrophobic effect. Further acetylation permeabilizes Rose Bengal for selective imaging, labeling and crosslinking aggregated proteome in live stressed cells. A combination of photo-chemical, tandem mass spectrometry and protein biochemistry characterizations reveal the complexity in photosensitizing pathways (Type I & II), modification sites and labeling mechanisms. The diverse labeling reaction types result in highly effective enrichment and identification of aggregated proteome. Finally, aggregated proteomics and interaction analyses thereby reveal extensive entangling of proteostasis network components mediated by HSP70 chaperone (HSPA1B) and active participation of autophagy pathway in combating proteasome inhibition. Overall, this work exemplifies the first photo-induced proximity labeling and crosslinking method (namely AggID) to profile intracellular aggregated proteome.
HostingRepository	iProX
AnnounceDate	2023-08-30
AnnouncementXML	Submission_2024-05-22_19:59:15.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nan Zhao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-29 23:46:32	ID requested
1	2023-08-29 23:46:53	announced
⏵ 2	2024-05-22 19:59:16	announced	2024-05-23: Update information.

Feng H, Zhao Q, Zhao N, Liang Z, Huang Y, Zhang X, Zhang L, Liu Y, A Cell-Permeable Photosensitizer for Selective Proximity Labeling and Crosslinking of Aggregated Proteome. Adv Sci (Weinh), 11(18):e2306950(2024) [pubmed]

submitter keyword: Aggregate, Proteome, Photosensitizer

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics
contact email	lihuazhang@dicp.ac.cn
lab head
Nan Zhao
contact affiliation	Dalian Institute of Chemical Physics
contact email	zhaonan@dicp.ac.cn
dataset submitter

iProX dataset URI