PXD044922-2

PXD044922 is an original dataset announced via ProteomeXchange.

Title	Proteasome resides in and dismantles plant heat stress granules constitutively
Description	Stress granules (SGs) are conserved reversible cytoplasmic condensates enriched with aggregation-prone proteins assembled in response to various stresses. Defects in disassembly have been associated with various neuro- and muscular degenerative diseases in humans. Plants appear to have efficient approaches to avoid the pathological conversion of SGs. However, how plants regulate SG dynamics is unclear. Here we show increase in either temperature or duration of heat stress reduces the molecular mobility of SG marker protein and suppresses SG clearance. Proteomics analysis and FRAP assays demonstrated 20S and 26S proteasomes as stable “core” components of SGs recruited early during the heat stress. Strikingly, while heat stress inhibits the activity of the overall proteasomes, it induces dramatic ubiquitylation of SG components and enhances the activities of SG-resident proteasomes, which degrade SG components even during the assembly phase. Their proteolytic activities enable the timely disassembly of SGs and secure the survival of plant cells during the recovery from heat stress.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:55:35.597.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhouli Xie
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-28 22:49:21	ID requested
1	2024-08-22 06:12:10	announced
⏵ 2	2024-10-22 06:55:35	announced	2024-10-22: Updated project metadata.

10.1016/J.MOLCEL.2024.07.033;

submitter keyword: Stress granules (SG), Proteasomes, Heat stress, SG disassembly

Wei Wang
contact affiliation	State Key Laboratory for Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
contact email	oneway1985@pku.edu.cn
lab head
Zhouli Xie
contact affiliation	Peking University
contact email	zhou_lixie@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD044922

PRIDE project URI

• PRIDE
  1. PXD044922
     1. Label: PRIDE project
     2. Name: Proteasome resides in and dismantles plant heat stress granules constitutively