PXD044917-1

PXD044917 is an original dataset announced via ProteomeXchange.

Title	Lymphatic platelet thrombosis inhibits fracture healing by decreased lymphatic transporting DAMPs in mice
Description	Lymphatic vessels (LVs) interdigitated with blood vessels, travel and form an extensive transport network in the musculoskeletal system. Blood vessels in bone regulate osteogenesis and hematopoiesis, however, whether LVs in bone affect fracture healing is unclear. Here, by near infrared indocyanine green lymphatic imaging (NIR-ICG), we examined lymphatic drainage function at the tibial fracture sites and found lymphatic drainage insufficiency (LDI) occurred as early as two weeks after fracture. Sufficient lymphatic drainage facilitates fracture healing. In addition, we identified that lymphatic platelet thrombosis (LPT) blocks the draining lymphoid sinus and LVs, caused LDI and then inhibited fracture healing, which can be rescued by a pharmacological approach. Moreover, unblocked lymphatic drainage inhibits neutrophils influx and induce M2-like macrophages palarization of hematoma niche to support osteoblast (OB) survival and bone marrow-derived mesenchymal stem cell (BMSC) proliferation via transporting damage-associated molecular patterns (DAMPs). These findings demonstrate that LPT limits fracture healing by blocking lymphatic drainage from transporting DAMPs. Together, these findings represent a novel way forward in the treatment of bone fracture healing.
HostingRepository	iProX
AnnounceDate	2023-08-28
AnnouncementXML	Submission_2024-10-27_19:53:42.083.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	EthanT Cheng
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-28 20:10:26	ID requested
⏵ 1	2024-10-27 19:53:42	announced

Dataset with its publication pending

submitter keyword: Lymphatic platelet thrombosis, lymphatic drainage, fracture healing, immunomodulation, DAMPs

Yongjun Wang
contact affiliation	Shanghai University of Traditional Chinese Medicine
contact email	wangyongjun@shutcm.edu.cn
lab head
EthanT Cheng
contact affiliation	Shanghai University of Traditional Chinese Medicine
contact email	ethantcheng@126.com
dataset submitter

iProX dataset URI