PXD044915-1

PXD044915 is an original dataset announced via ProteomeXchange.

Title	Ustilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities
Description	Diversification of effector function, driven by a co-evolutionary arms race, enables pathogens to establish compatible interactions with hosts. Structurally conserved plant pathogenesis-related PR-1 and PR-1-like (PR-1L) proteins are involved in plant defense and fungal virulence, respectively. It is unclear how fungal PR-1L counters plant defense. Here, we show that Ustilago maydis UmPR-1La and yeast ScPRY1, with conserved phenolic resistance functions, are Ser/Thr-rich region-mediated cell-surface localization proteins. However, UmPR-1La has gained specialized activity in sensing phenolics and eliciting hyphal-like formation to guide fungal growth in plants. Additionally, U. maydis hijacks maize cathepsin B-like 3 (CatB3) to release functional CAPE-like peptides by cleaving UmPR-1La’s conserved CNYD motif, subverting plant CAPE-primed immunity and promoting fungal virulence. Surprisingly, CatB3 avoids cleavage of plant PR-1s, despite the presence of the same conserved CNYD motif. Our work highlights that UmPR-1La has acquired additional dual roles to suppress plant defense and sustain the infection process of fungal pathogens.
HostingRepository	PRIDE
AnnounceDate	2023-08-29
AnnouncementXML	Submission_2023-08-28_20:02:04.839.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuan-ChihHsu
SpeciesList	scientific name: Ustilago maydis (Smut fungus); NCBI TaxID: 5270;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-28 19:45:19	ID requested
⏵ 1	2023-08-28 20:02:05	announced
2	2024-10-22 06:02:03	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Ustilago maydis, CAPE-like, fungal virulence, Cathepsin B-like 3, PRY1, phenolic resistance, PR-1-like, CNYD motif

Lay-SunMa
contact affiliation	Institute of Plant and Microbial Biology, Academia Sinica
contact email	laysunma@gate.sinica.edu.tw
lab head
Chuan-ChihHsu
contact affiliation	Academia Sinica
contact email	without1102@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD044915

PRIDE project URI

• PRIDE
  1. PXD044915
     1. Label: PRIDE project
     2. Name: Ustilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities