PXD044876-1

PXD044876 is an original dataset announced via ProteomeXchange.

Title	Molecular mechanism of distinct chemokine engagement and functional divergence of the human Duffy antigen receptor
Description	The Duffy antigen receptor, also known as FY glycoprotein or CD234, is a seven transmembrane (7TM) protein expressed primarily at the surface of red blood cells, and it displays strikingly promiscuous binding to multiple pro- and anti-inflammatory chemokines. Here, we comprehensively profile transducer-coupling for this receptor including G-proteins, GRKs, and β-arrestins to unequivocally establish its dramatic functional divergence, and discover potential non-canonical signaling pathways using a global phosphoproteomics analysis. we present a comprehensive profiling of transducer-coupling for this receptor using cell-based assays, and a global phosphoproteomics screen to identify novel, non-canonical downstream signaling pathways. We prepared samples under unstimulated and CCL7-stimulated conditions, generated proteolytic fragments by trypsin digestion, enriched for phosphorylated peptides using TiO2 beads, and then identified the phosphorylated peptides using liquid chromatography-tandem MS (LC-MS/MS).
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:45:32.923.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	gagan jhingan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-26 01:38:30	ID requested
⏵ 1	2024-10-17 02:45:33	announced

10.1016/j.cell.2024.07.005;

submitter keyword: Duffy antigen receptor, G protein-coupled receptors, chemokine receptors, cellular signaling, cryogenic-electron microscopy, phosphoproteomics

Dr. Arun
contact affiliation	Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India
contact email	arshukla@iitk.ac.in
lab head
gagan jhingan
contact affiliation	VProteomics
contact email	gaganjhingan@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044876
     1. Label: PRIDE project
     2. Name: Molecular mechanism of distinct chemokine engagement and functional divergence of the human Duffy antigen receptor