PXD044872-1

PXD044872 is an original dataset announced via ProteomeXchange.

Title	Feed-forward stimulation of CAMK2 by the oncogenic pseudokinase PEAK1 generates an ‘actionable’ signalling axis in triple negative breast cancer
Description	To characterize the interactomes of PEAK1 and PEAK2 homodimers, and the PEAK1/PEAK2 heterodimer, we applied bimolecular complementation affinity purification coupled with tandem mass spectrometry (BiCAP-MS/MS), a recently-developed technique that specifically identifies the interactors of any pair of interacting proteins while excluding those binding to individual components. Appropriate pairs of V1- and V2-tagged proteins were co-transfected into HEK293T cells, and then specific dimeric complexes were affinity purified with GFP-Trap nanobody and associated proteins identified by LC-MS/MS. Proteins exhibiting significantly increased abundance in particular PEAK dimers versus the Venus control were identified by label-free quantitative MS analysis, and are presented in volcano plots.
HostingRepository	PRIDE
AnnounceDate	2025-01-04
AnnouncementXML	Submission_2025-01-04_07:25:18.690.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xue Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-25 18:42:42	ID requested
⏵ 1	2025-01-04 07:25:19	announced

10.1101/2024.02.14.580406;

Yang X, Ma X, Zhao T, Croucher DR, Nguyen EV, Clark KC, Hu C, Latham SL, Bayly-Jones C, Nguyen VCB, Budnar S, Shin S, Nguyen LK, Cotton TR, Chueh AC, Sian TCLK, Stratton MM, Ellisdon AM, Daly RJ, Feed-forward stimulation of CAMK2 by the oncogenic pseudokinase PEAK1 generates a therapeutically "actionable" signalling axis in triple negative breast cancer. bioRxiv, ():(2024) [pubmed]

submitter keyword: MS, dimerization,BiCAP, HEK293T, interactome

Roger John Daly
contact affiliation	Cancer Program, Monash Biomedicine Discovery Institute, Clayton, VIC 3800, Australia. Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC 3800, Australia.
contact email	roger.daly@monash.edu
lab head
Xue Yang
contact affiliation	Cancer Program, Monash Biomedicine Discovery Institute, Clayton, VIC 3800, Australia. Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC 3800, Australia.
contact email	xue.yang@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD044872

PRIDE project URI

• PRIDE
  1. PXD044872
     1. Label: PRIDE project
     2. Name: Feed-forward stimulation of CAMK2 by the oncogenic pseudokinase PEAK1 generates an ‘actionable’ signalling axis in triple negative breast cancer