PXD044866-1

PXD044866 is an original dataset announced via ProteomeXchange.

Title	FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes
Description	Cold stimulation dynamically remodels mitochondria in brown adipose tissue (BAT) to facilitate non-shivering thermogenesis in mammals, but what regulates mitochondrial plasticity is poorly understood. Comparing mitochondrial proteomes in response to cold identify FAM210A as a cold-inducible mitochondrial inner membrane protein. Adipocyte-specific constitutive knockout of Fam210a (Fam210aAKO) disrupts mitochondrial cristae structure and diminishes the thermogenic activity of BAT, rendering the Fam210aAKO mice lethal hyperthermia under acute cold exposure. Induced knockout of Fam210a in adult adipocytes (Fam210aiAKO) does not affect steady-state, non-thermogenic mitochondria under thermoneutrality, but impairs cold-induced mitochondrial remodeling, leading to progressive loss of cristae and reduction of mitochondrial density. Proteomics reveals an association between FAM210A and OPA1, whose cleavage governs cristae dynamics and mitochondrial remodeling. Mechanistically, FAM210A interacts with mitochondrial protease YME1L and modulates its activity toward OMA1 and OPA1 cleavage. These data establish FAM210A as a key regulator of mitochondrial cristae remodeling in BAT and shed light on the mechanism underlying mitochondrial plasticity in response to cold.
HostingRepository	PRIDE
AnnounceDate	2023-08-26
AnnouncementXML	Submission_2023-08-25_20:02:49.391.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JiaminQiu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-25 14:18:54	ID requested
⏵ 1	2023-08-25 20:02:49	announced
2	2024-10-22 06:01:50	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: LC-MSMS,mouse, brown adipocyte, cold, brown fat, mitochondria, FAM210A

ShihuanKuang
contact affiliation	Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA Center for Cancer Research Purdue University, West Lafayette, IN 47907, USA
contact email	skuang@purdue.edu
lab head
JiaminQiu
contact affiliation	Purdue University
contact email	qiu138@purdue.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD044866

PRIDE project URI

• PRIDE
  1. PXD044866
     1. Label: PRIDE project
     2. Name: FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes