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PXD044856-1

PXD044856 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGermline-specific RNA helicase DDX4 forms cytoplasmic granules in cancer cells and promotes tumor growth
DescriptionCancer cells undergo major epigenetic alterations and transcriptome changes, including ectopic expression of many tissue- and cell type-specific genes. Here we show that the germline-specific RNA helicase DDX4 forms germ granule-like cytoplasmic ribonucleoprotein (RNP) granules in various human and mouse tumors, but not in cultured cancer cells. The DDX4-compexes in tumors contain RNA-binding proteins and splicing regulators, including many known germ granule components. The deletion of DDX4 in cancer cells induces transcriptome imbalance and affects the alternative splicing landscape of a large number of genes with reported functions in cancer growth and invasiveness, leading to their compromised capability to form xenograft tumors. Importantly, high expression of DDX4-positive granules is associated with poor survival in patients with squamous cell carcinoma of the head and neck, as well as with the histological grade of prostate cancer. Taken together, these results show that the formation of germ granule-resembling DDX4 granules have prognostic value, and they are involved in transcriptome control in cancer cells to promote malignant properties.
HostingRepositoryPRIDE
AnnounceDate2024-06-10
AnnouncementXMLSubmission_2024-06-10_01:00:40.662.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLin Ma
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-25 06:10:27ID requested
12024-06-10 01:00:41announced
22024-07-03 03:51:37announced2024-07-03: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: cancer,DDX4, PC3 cells
Contact List
Noora Kotaja
contact affiliationInstitute of Biomedicine, University of Turku, Finland
contact emailnookot@utu.fi
lab head
Lin Ma
contact affiliationUniversity of Turku
contact emailmalin.ml@outlook.com
dataset submitter
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Dataset FTP location
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