PXD044850-2

PXD044850 is an original dataset announced via ProteomeXchange.

Title	Transport properties of canonical PIN proteins and the role of the loop in auxin transport
Description	Auxin is a pivotal plant hormone that controls virtually all aspects of plant growth and development. Auxin is transported in plants in a polar fashion and PIN-FORMED (PIN) transporters are the key players in this process. Canonical PINs possess a long cytosolic loop that is subject to regulation by AGC1 and AGC3 kinases. To characterize the transporters, they are expressed in Xenopus laevis oocytes alone or with the different activating kinases. Since transport rates always depend on the amount of active transporter in the membrane it is paramount to ensure that the levels of transporter, kinase and degree of phosphorylation are similar to be able to compare transport rates directly. To this end we expressed PIN1, PIN2 and PIN3 either alone or with D6PK (an AGC1 kinase) or PID (an AGC3 kinase) in oocytes. The protein levels and the degree of phosphorylation of the loop were established and found to be comparable between PINs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:56:03.336.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Mergner
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-25 02:40:28	ID requested
1	2024-08-23 00:35:11	announced
⏵ 2	2024-10-22 06:56:03	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Phosphoproteomics,PIN proteins, Auxin transport

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich, Germany
contact email	kuster@tum.de
lab head
Julia Mergner
contact affiliation	Chair of Proteomics and Bioanalytics, TU Munich
contact email	julia.mergner@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044850
     1. Label: PRIDE project
     2. Name: Transport properties of canonical PIN proteins and the role of the loop in auxin transport