PXD044784-1

PXD044784 is an original dataset announced via ProteomeXchange.

Title	Comprehensive proteomic analysis of JC polyomavirus-infected human astrocytes and their extracellular vesicles
Description	JC polyomavirus (JCPyV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a devastating demyelinating disease of the central nervous system that results in the widespread formation of lesions across the brain parenchyma. The virus is opportunistic and remains in a benign state in the kidneys and lymphoid organs of more than half of the global human adult population. However, in rare cases of severe or selective immune suppression, JCPyV can establish a lytic infection of glial cells in the brain. While PML has traditionally been characterized as a lytic infection of oligodendrocytes, more recent findings suggest an important role for astrocytes during the initial stages of disease. Because of the exceptional species and tissue specificity of the virus, appropriate models of JCPyV infection in the brain are lacking, thus hampering progress towards the development of novel antiviral strategies and biomarkers of disease activity. Towards the aim of biomarker development extracellular vesicles (EVs) were isolated from JCPyV-infected and mock-infected human iPSC-derived astrocytes and analyzed by LC-MS/MS. As a inflammatory control, EVs were also isolated from cytokine-stimulated iPSC-derived astrocytes. We demonstrate that the proteomic signature associated with EVs from JCPyV-infected astrocytes reflect what was observed on a cellular level for infected astrocytes while being strikingly different from that of EVs generated under inflammatory conditions.
HostingRepository	PRIDE
AnnounceDate	2023-08-28
AnnouncementXML	Submission_2023-08-28_00:27:45.269.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	LariseOberholster
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: JC polyomavirus; NCBI TaxID: 10632;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-23 02:49:45	ID requested
⏵ 1	2023-08-28 00:27:45	announced
2	2024-10-22 06:01:54	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: PML, astrocytes,hiPSC, model, extracellular vesicles, JCPyV

Renaud A. DuPasquier
contact affiliation	Laboratory of Neuroimmunology, Neuroscience Research Centre, Department of Clinical Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne, Lausanne, Switzerland Service of Neurology, Department of Clinical Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne, Lausanne, Switzerland
contact email	Renaud.Du-Pasquier@chuv.ch
lab head
LariseOberholster
contact affiliation	Lausanne University Hospital and University of Lausanne
contact email	lariseoberholster@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD044784

PRIDE project URI

• PRIDE
  1. PXD044784
     1. Label: PRIDE project
     2. Name: Comprehensive proteomic analysis of JC polyomavirus-infected human astrocytes and their extracellular vesicles