PXD044783-1

PXD044783 is an original dataset announced via ProteomeXchange.

Title	Systematic characterization of site-specific proline hydroxylation using hydrophilic interaction chromatography and mass spectrometry (RCC4)
Description	To improve understanding of the role of site-specific proline hydroxylation in controlling protein function, we have developed a robust workflow for the identification of proline hydroxylation sites in proteins using a combination of hydrophilic interaction chromatography (HILIC) enrichment and high-resolution nano-Liquid Chromatography-Mass Spectrometry (LC-MS). Using this approach, together with refining and filtering parameters during data analysis, by combining the results from cell lines being treated with either the prolyl hydroxylase inhibitor Roxadustat (FG-4592, FG) or the proteasome inhibitor MG-132 (MG), or DMSO, a total of 4,993 and 3,247 proline hydroxylation sites were identified in HEK293 (PT10822) and RCC4 (PT10402) samples.
HostingRepository	PRIDE
AnnounceDate	2025-11-30
AnnouncementXML	Submission_2025-11-29_18:20:31.292.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hao Jiang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-23 02:36:56	ID requested
⏵ 1	2025-11-29 18:20:31	announced

10.7554/ELIFE.108128.1;

submitter keyword: HILIC, proteomics,Hydroxylation, mass spectrometry, proline

Angus Lamond
contact affiliation	Molecular Cell and Developmental Biology, School of Life Sciences, University of Dundee
contact email	a.i.lamond@dundee.ac.uk
lab head
Hao Jiang
contact affiliation	University of Dundee
contact email	h.y.jiang@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD044783

PRIDE project URI

• PRIDE
  1. PXD044783
     1. Label: PRIDE project
     2. Name: Systematic characterization of site-specific proline hydroxylation using hydrophilic interaction chromatography and mass spectrometry (RCC4)