PXD044765-1

PXD044765 is an original dataset announced via ProteomeXchange.

Title	BCL2L13 regulates sarcoplasmic reticulum-mitochondria calcium flux in skeletal muscle
Description	The physical connection between mitochondria and endoplasmic or sarcoplasmic reticulum is an essential signaling hub to ensure organelle and cellular functions. In skeletal muscle, ER/SR-mitochondria calcium (Ca2+) signaling is crucial to maintain cellular homeostasis during physical activity. High expression of BCL2L13, a member of the BCL-2 family, was suggested as an adaptive response in endurance-trained human subjects. The aim of this study was to describe the molecular and physiological functions of BCL2L13. Using a zebrafish knockout model, we assessed the physiological changes, alterations of skeletal muscle structure, differences in the muscle proteome, and mitochondrial metabolism caused by the loss of Bcl2l13. We used cellular models to define the subcellular location and the mechanistic role of Bcl2l13. The loss of Bcl2l13 in zebrafish decreased swimming capacity and activity. Skeletal muscle fast fiber cross sectional area was reduced in knockout fish. Muscle proteome uncovered changes in protein turnover, transmembrane transport and expression of Ca2+ signaling proteins compared to wild type fish.
HostingRepository	PRIDE
AnnounceDate	2024-07-25
AnnouncementXML	Submission_2024-07-25_09:48:37.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfredo Quadroni
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-23 01:22:21	ID requested
⏵ 1	2024-07-25 09:48:37	announced
2	2024-10-22 06:51:24	announced	2024-10-22: Updated project metadata.

10.1016/J.ISCI.2024.110510;

submitter keyword: Endoplasmic reticulum mitochondria contact sites ERMCs, Mitochondria-associated membranes MAMs, C2C12, Bcl-2 family, Mitochondria-ER contact sites MERCs, zebrafish

Francesca Amati
contact affiliation	Aging and Muscle Metabolism Lab, Department of Biomedical Sciences & Service of Endocrinology, Diabetes and Metabolism, Faculty of Biology and Medicine, Lausanne University Hospital and University of Lausanne, Bugnon 7, 1005 Lausanne, Switzerland
contact email	Francesca.amati@unil.ch
lab head
Manfredo Quadroni
contact affiliation	University of Lausanne
contact email	manfredo.quadroni@unil.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD044765

PRIDE project URI

• PRIDE
  1. PXD044765
     1. Label: PRIDE project
     2. Name: BCL2L13 regulates sarcoplasmic reticulum-mitochondria calcium flux in skeletal muscle