PXD044747-1

PXD044747 is an original dataset announced via ProteomeXchange.

Title	Identification of small proteins in nodules of Robinia pseudoacacia
Description	Robinia pseudoacacia L. (black locust) is capable of nitrogen fixation in association with rhizobia. Global transcriptional analysis in Robinia pseudoacacia nodules revealed the presence of three highly abundant small transcripts that may encode small proteins. The high number of proline and glycine residues is the most interesting feature of these proteins. A gelfree bottom up proteomics approach was used for the direct detection of the predicted small proteins. Crude protein extracts were obtained from nodules of wild Robinia (Robinia pseudoacacia). The endoproteases AspN and α-lytic protease were selected for protein digestion. One of these small proteins has been experimentally identified by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2026-03-06
AnnouncementXML	Submission_2026-03-06_05:55:36.728.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Kucklick
SpeciesList	scientific name: Robinia pseudoacacia; NCBI TaxID: NEWT:35938;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-22 11:55:03	ID requested
⏵ 1	2026-03-06 05:55:37	announced

Dataset with its publication pending

submitter keyword: None

Susanne Engelmann
contact affiliation	CPRO, Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany
contact email	susanne.engelmann@helmholtz-hzi.de
lab head
Martin Kucklick
contact affiliation	TU Braunschweig Institut für Mikrobiologie Spielmannstr. 7 Germany
contact email	m.kucklick@tu-braunschweig.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD044747

PRIDE project URI

• PRIDE
  1. PXD044747
     1. Label: PRIDE project
     2. Name: Identification of small proteins in nodules of Robinia pseudoacacia