PXD044706-1

PXD044706 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Hydrogen Deuterium Exchange of PIKfyve construct with PI3P vesicles and inhibitors
Description	We used HDXMS to study the confomational changes induced in a PIKfyve construct upon binding PI3P membrane and a variety of inhibitors. Here we showed a significant protection in deuterium exchange upon binding all inhibitors, and regions of protection and exposure upon binding PI3P membrane.
HostingRepository	PRIDE
AnnounceDate	2025-10-15
AnnouncementXML	Submission_2025-10-15_15:50:53.878.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Burke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	impact HD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-21 11:46:01	ID requested
⏵ 1	2025-10-15 15:50:54	announced

Publication List

10.1083/jcb.202408158;
Pemberton JG, Barlow-Busch I, Jenkins ML, Parson MAH, Sarnyai F, Bektas SN, Kim YJ, Heuser JE, Burke JE, Balla T, An advanced toolset to manipulate and monitor subcellular phosphatidylinositol 3,5-bisphosphate. J Cell Biol, 224(6):(2025) [pubmed]

10.1083/jcb.202408158;

Pemberton JG, Barlow-Busch I, Jenkins ML, Parson MAH, Sarnyai F, Bektas SN, Kim YJ, Heuser JE, Burke JE, Balla T, An advanced toolset to manipulate and monitor subcellular phosphatidylinositol 3,5-bisphosphate. J Cell Biol, 224(6):(2025) [pubmed]

Keyword List

submitter keyword: HDXMS, APY0201, YM-201636, MOMIPP, PIKfyve, PI3P, Apilimod

submitter keyword: HDXMS, APY0201, YM-201636, MOMIPP, PIKfyve, PI3P, Apilimod

Contact List

Dr. John E. Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
lab head
John Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD044706
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD044706

PRIDE project URI

Repository Record List

[ + ]