PXD044656-1

PXD044656 is an original dataset announced via ProteomeXchange.

Title	The alternative Nucleosome Remodeling Factor complex sustains acute myeloid leukemia by regulating the accessibility of insulator regions
Description	Efficient treatment of Acute Myeloid Leukemia (AML) patients remains a challenge despite recent advances. Here using a CRISPRi screen targeting chromatin factors, we identified BPTF as an essential regulator of AML cell survival. We demonstrate that BPTF forms an alternative NURF chromatin remodeling complex with SMARCA5 and BAP18, which regulates the accessibility of a large set of insulator regions in leukemic cells. This ensures efficient CTCF binding and boundary formation between topologically associated domains that is essential for maintaining the leukemic transcriptional programs. We also demonstrate that the well-studied PHD2-BROMO chromatin reader modules of BPTF, while contributing to complex recruitment to chromatin, are dispensable for leukemic cell growth. Taken together, our results uncover how the alternative NURF complex contributes to leukemia and provide a rationale for its targeting in AML.
HostingRepository	PRIDE
AnnounceDate	2023-11-12
AnnouncementXML	Submission_2023-11-12_02:45:13.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PavelShliaha
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-18 13:12:22	ID requested
⏵ 1	2023-11-12 02:45:13	announced
2	2023-11-14 07:26:38	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:13:54	announced	2024-10-22: Updated project metadata.

submitter keyword: BPTF,human, AML

PavelShliaha
contact affiliation	Head of MRC LMS proteomics core
contact email	pavel.shliaha@gmail.com
lab head
PavelShliaha
contact affiliation	MRC London Institute of Medical Sciences proteomics and metabolomics core
contact email	pavel.shliaha@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044656
     1. Label: PRIDE project
     2. Name: The alternative Nucleosome Remodeling Factor complex sustains acute myeloid leukemia by regulating the accessibility of insulator regions