PXD044644

PXD044644 is an original dataset announced via ProteomeXchange.

Title	Neutrophil extracellular traps melt Peyer’s patches following stroke and myocardial infarction
Description	Sterile tissue injury after stroke causes lymphocyte contraction in lymphoid tissues and may decrease circulating IgA-levels. Intestinal Peyer’s patches (PP) harbor large numbers of IgA+ B cell precursors and plasma cells. Whether and how tissue injury triggers PP-B cell death, thereby mediating IgA-loss, is unknown. We found decreased circulating IgA levels in stroke and myocardial infarction patients. Experimental stroke and myocardial infarction in mice phenocopied the human situation. Decreased plasma and fecal IgA were accompanied by rapid and macroscopic shrinkage of PP caused by substantial losses of PP-resident IgA+ precursors and plasma cells in mice. Tissue injury induced neutrophil activation endowed with the release of toxic neutrophil extracellular traps (NETs). Antibody-mediated or genetically- induced neutrophil loss, digestion of NETs, or inhibition of their release by the Gasdermin D blockade completely prevented lymphocyte loss and PP shrinkage. We also identified NETs in the plasma of stroke and myocardial infarction patients. Hence, tissue injury induces systemic NET-release, which might be targeted to maintain immune homeostasis at mucosal barriers.
HostingRepository	PRIDE
AnnounceDate	2024-02-28
AnnouncementXML	Submission_2024-02-28_06:25:00.619.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susmita Ghosh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-18 08:36:46	ID requested
⏵ 1	2024-02-28 06:25:01	announced

10.21203/RS.3.RS-3030780/V1;

submitter keyword: Stroke, neutrophil extracellular traps, immunosuppression, lymphocytes, myocardial infarction, Peyer’s patches

Prof. Dr. Albert Sickmann
contact affiliation	President Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany P: +49 (0)2 31.13 92-100 F: +49 (0)2 31.13 92-200 albert.sickmann@isas.de www.isas.de twitter.com/isas_Leibniz
contact email	albert.sickmann@isas.de
lab head
Susmita Ghosh
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact email	susmita.ghosh@isas.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD044644

PRIDE project URI

• PRIDE
  1. PXD044644
     1. Label: PRIDE project
     2. Name: Neutrophil extracellular traps melt Peyer’s patches following stroke and myocardial infarction