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PXD044644 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNeutrophil extracellular traps melt Peyer’s patches following stroke and myocardial infarction
DescriptionSterile tissue injury after stroke causes lymphocyte contraction in lymphoid tissues and may decrease circulating IgA-levels. Intestinal Peyer’s patches (PP) harbor large numbers of IgA+ B cell precursors and plasma cells. Whether and how tissue injury triggers PP-B cell death, thereby mediating IgA-loss, is unknown. We found decreased circulating IgA levels in stroke and myocardial infarction patients. Experimental stroke and myocardial infarction in mice phenocopied the human situation. Decreased plasma and fecal IgA were accompanied by rapid and macroscopic shrinkage of PP caused by substantial losses of PP-resident IgA+ precursors and plasma cells in mice. Tissue injury induced neutrophil activation endowed with the release of toxic neutrophil extracellular traps (NETs). Antibody-mediated or genetically- induced neutrophil loss, digestion of NETs, or inhibition of their release by the Gasdermin D blockade completely prevented lymphocyte loss and PP shrinkage. We also identified NETs in the plasma of stroke and myocardial infarction patients. Hence, tissue injury induces systemic NET-release, which might be targeted to maintain immune homeostasis at mucosal barriers.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSusmita Ghosh
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-18 08:36:46ID requested
12024-02-28 06:25:01announced
Publication List
Keyword List
submitter keyword: Stroke, neutrophil extracellular traps, immunosuppression, lymphocytes, myocardial infarction, Peyer’s patches
Contact List
Prof. Dr. Albert Sickmann
contact affiliationPresident Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany P: +49 (0)2 31.13 92-100 F: +49 (0)2 31.13 92-200 albert.sickmann@isas.de www.isas.de twitter.com/isas_Leibniz
contact emailalbert.sickmann@isas.de
lab head
Susmita Ghosh
contact affiliationLeibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact emailsusmita.ghosh@isas.de
dataset submitter
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Dataset FTP location
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