PXD044629 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | hTERT mesenchymal stem cells (MSCs), Stem cells EVs LC-MS/MS |
Description | As the economic challenges associated with vision impairment and ocular injuries continue to escalate, there exists an urgent need to explore innovative remedies for these conditions. Extracellular Vesicles (EVs), containing a diverse array of biological components such as nucleic acids, proteins, and lipids, have garnered substantial scientific support for their healing and immune-regulating attributes in addressing various disorders. Nevertheless, an area that remains relatively uncharted is the potential reparative impact of stem cell-derived EVs within the realm of ocular damage or disease. Furthermore, prevailing literature predominantly focuses on EVs derived from primary stem cells, neglecting a comprehensive assessment of EVs derived from hTERT-immortalized stem cells. Through our extensive EV production platform, we have consistently generated EVs sourced from hTERT mesenchymal stem cells (MSCs) and have employed diverse techniques to characterize and profile their cargo contents. Leveraging established cellular assays, we have conducted a comparative analysis of these EVs' effects on both intact and impaired retinal pigment epithelial cells. To the best of our knowledge, this marks the inaugural study demonstrating the feasibility of consistent, large-scale production of hTERT MSC EVs and exploring their potential regenerative properties against compromised ocular cells. The outcomes of our investigations affirm that hTERT MSCs elicit multiple reparative influences in vitro, underscoring their promise not only as a viable substitute for primary MSC-derived EVs but also as a prospective innovative therapeutic agent for addressing ocular injuries. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-16 |
AnnouncementXML | Submission_2024-06-15_22:54:43.473.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fatah Kashanchi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-18 08:32:27 | ID requested | |
⏵ 1 | 2024-06-15 22:54:44 | announced | |
Publication List
Hindle J, Williams A, Kim Y, Kim D, Patil K, Khatkar P, Osgood Q, Nelson C, Routenberg DA, Howard M, Liotta LA, Kashanchi F, Branscome H, hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells. Cells, 13(10):(2024) [pubmed] |
10.3390/cells13100861; |
Keyword List
submitter keyword: Stem cells, EVs, LC-MS/MS |
Contact List
Fatah Kashanchi |
contact affiliation | Professor, Virology Director of the Laboratory of Molecular Virology |
contact email | fkashanc@gmu.edu |
lab head | |
Fatah Kashanchi |
contact affiliation | George Mason University |
contact email | fkashanc@gmu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044629
- Label: PRIDE project
- Name: hTERT mesenchymal stem cells (MSCs), Stem cells EVs LC-MS/MS