PXD044616-1

PXD044616 is an original dataset announced via ProteomeXchange.

Title	Characterization of the regulatory AAA-ATPase subunit Rpt3 in Plasmodium berghei as an activator of Protein Phosphatase 1
Description	The 26S proteasome is the main proteolytic machine involved in protein degradation, contributing to homeostasis or stress response of eukaryotic cells. This macromolecular complex, consisting of a 20S core particle assembled with one or two 19S regulatory particles, is highly regulated by phos-phorylation. Here we described the Plasmodium berghei (Pb) proteasome AAA-ATPase regulatory subunit Rpt3 and showed that it binds to protein phosphatase 1 catalytic subunit (PP1c), one of the major and essential parasite phosphatases. In addition, PbRpt3 protein was able to enhance the activity of PP1c both in vitro and in a heterologous model of Xenopus oocytes. Using mutagen-esis approaches, we observed that the RVXF motifs of PbRpt3 are involved in its binding and reg-ulatory function. Further use of Xenopus oocytes in combination with PbRpt3 protein mutated for amino acids predicted to be involved in ATP binding, suggested that the binding capacity of PbRpt3 to ATP may also contribute to its phosphatase-regulatory activity. In Pb, reverse genetic studies suggested an essential role for PbRpt3 since no viable knock-out line could be obtained. Finally, immunoprecipitation assays followed by mass spectrometry analyses using transgenic PbRpt3-tagged parasites confirmed that PbRpt3 belongs to the 19S regulatory particle of the pro-teasome and revealed potential interactions with proteins previously shown to be involved in membrane phospholipid binding.
HostingRepository	PRIDE
AnnounceDate	2025-12-03
AnnouncementXML	Submission_2025-12-03_11:22:23.890.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-18 05:06:02	ID requested
⏵ 1	2025-12-03 11:22:24	announced

Dataset with its publication pending

submitter keyword: proteasome, Rpt3, Protein Phosphatase 1, 19S regulatory particle,LCMS, Plasmodium

Ida Chiara Guerrera
contact affiliation	Proteomics platform Necker, Université Paris Cité - Structure Fédérative de Recherche Necker, INSERM US24/CNRSUAR3633, Paris 75015, France.
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD044616

PRIDE project URI

• PRIDE
  1. PXD044616
     1. Label: PRIDE project
     2. Name: Characterization of the regulatory AAA-ATPase subunit Rpt3 in Plasmodium berghei as an activator of Protein Phosphatase 1