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PXD044576-1

PXD044576 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEndoGenius: Optimized neuropeptide identification from mass spectrometry datasets
DescriptionNeuropeptides represent a unique class of signaling molecules that have garnered much attention but require special consideration when gleaning identifications from mass spectra. With highly variable sequence lengths, neuropeptides must by analyzed in their endogenous state. Further, neuropeptides share great homology within families, differing by as little as a single residue, complicating even routine analyses and necessitating optimized computational strategies for identifications. We present EndoGenius, a database searching strategy designed specifically for elucidating neuropeptide identifications from mass spectra by leveraging optimized peptide-spectrum matching approaches, an expansive motif database, and a novel scoring algorithm to achieve broader representation of the neuropeptidome and minimize re-identification. This work describes an algorithm capable of reporting more neuropeptide identifications at 1% false discovery rate than PEAKS DB in five Callinectes sapidus neuronal tissue types.
HostingRepositoryMassIVE
AnnounceDate2024-02-08
AnnouncementXMLSubmission_2024-02-08_11:04:46.458.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLauren Fields
SpeciesList scientific name: Callinectes sapidus; common name: blue crab; NCBI TaxID: 6763;
ModificationListOxidation; Glu->pyro-Glu; Gln->pyro-Glu; Amidated; sulfo+amino
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-14 16:22:28ID requested
12024-02-08 11:04:46announced
Publication List
no publication
Keyword List
submitter keyword: neuropeptide, endogenius, homology, peptide, mass spectrometry, database searching, FDR, endogenous, peptidomics, digest-free
Contact List
Lingjun Li
contact affiliationUniversity of Wisconsin-Madison
contact emaillingjun.li@wisc.edu
lab head
Lauren Fields
contact affiliationUW Madison
contact emaillawashburn@wisc.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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