PXD044550-1

PXD044550 is an original dataset announced via ProteomeXchange.

Title	Coordinated miRNA activity mediates the cellular transition into dormancy during embryonic diapause
Description	Dormancy is a key feature of stem cell function in adult tissues as well as embryonic cells in the context of diapause. The establishment of dormancy is an active process that involves extensive transcriptional, epigenetic, and metabolic rewiring. How these processes are coordinated to successfully transition cells to the resting dormant state is not known. Here we show that microRNA activity, which is normally dispensable for pre-implantation development, is essential for the adaptation of early mouse embryos to the dormant state of diapause. In particular, the pluripotent epiblast depends on miRNA activity, the absence of which results in loss of pluripotency and embryo collapse. Through tissue-specific small RNA profiling of single embryos and computational analyses of miRNA targets, we identified the miRNA-protein network of diapause. Individual miRNA function contributes to combinatorial regulation by the network of most notably RNA processing and chromatin modifier proteins. Without miRNAs, multiple nuclear and cytoplasmic bodies show aberrant expression and structure in normal ESCs and fail to reorganize in response to stress. We find extensive alternative splicing in wild-type, but not miRNA-deficient ESCs, of cell cycle and metabolic regulators. Our results reveal that miRNAs are critical for the transcriptional and structural rewiring of pluripotent cells in response to stress and to establish dormancy in the pluripotent state.
HostingRepository	PRIDE
AnnounceDate	2024-03-26
AnnouncementXML	Submission_2024-03-26_01:58:20.455.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Meierhofer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-14 01:20:21	ID requested
⏵ 1	2024-03-26 01:58:20	announced
2	2024-10-22 06:34:34	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: embryonic diapause

Aydan Bulut-Karslioglu
contact affiliation	Research Group Leader Bulut-Karslioglu lab Dept. of Genome Regulation Max Planck Institute for Molecular Genetics Ihnestrasse 63-73 14195 Berlin GERMANY
contact email	karsliog@molgen.mpg.de
lab head
David Meierhofer
contact affiliation	Mass Spectrometry Facility MPIMG
contact email	meierhof@molgen.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD044550

PRIDE project URI

• PRIDE
  1. PXD044550
     1. Label: PRIDE project
     2. Name: Coordinated miRNA activity mediates the cellular transition into dormancy during embryonic diapause