PXD044545-1

PXD044545 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Improved drug-target deconvolution with PISA-DIA using an extended, overlapping temperature gradient
Description	For target-engagement applications, a comparative analysis was performed to assess the suitability, ease of implementation and cost-effectiveness between Cellular Thermal Shift Assay (CETSA) and Proteome Integral Solubility Alteration (PISA). Human multiple myeloma cells U266B1 cell lysate was treated with either 1 μM BCL-XL inhibitor or DMSO as a vehicle to perform i) label-free PISA approach to analyse each PISA pool by data-independent acquisition (DIA) approach (PISA-DIA-MS) and ii) traditional TMT labelled proteome-wide CETSA approach (CETSA-TMT-MS).
HostingRepository	PRIDE
AnnounceDate	2024-05-20
AnnouncementXML	Submission_2024-05-20_15:53:23.871.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Dagley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-13 22:40:31	ID requested
⏵ 1	2024-05-20 15:53:24	announced

Publication List

10.1002/PMIC.202300644;

10.1002/PMIC.202300644;

Keyword List

submitter keyword: Data independent acquisition (DIA), Thermal proteome profiling,Proteomics, Mass spectrometry, Target engagement, Protein stability, Proteome integral solubility alternation

submitter keyword: Data independent acquisition (DIA), Thermal proteome profiling,Proteomics, Mass spectrometry, Target engagement, Protein stability, Proteome integral solubility alternation

Contact List

Dr. Laura Dagley
contact affiliation	Advanced Technology and Biology Division, The Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, Australia
contact email	dagley.l@wehi.edu.au
lab head
Laura Dagley
contact affiliation	WEHI
contact email	dagley.l@wehi.edu.au
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD044545
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD044545

PRIDE project URI

Repository Record List

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