PXD044528-1

PXD044528 is an original dataset announced via ProteomeXchange.

Title	Rab30 regulates lipid homeostasis in the hepatocyte during fasting
Description	In order to facilitate inter-tissue communication and exchange of proteins, lipoproteins, and metabolites with the circulation, hepatocytes have an intricate and efficient intracellular trafficking system regulated by small Rab GTPases. Rab30, a putative Golgi-localized Rab GTPase, is induced in the mouse liver by fasting and its expression is further amplified in liver-specific carnitine palmitoyltransferase 2 knockout mice (Cpt2L-/-) that lack the ability to oxidize fatty acids in a Pparα-dependent manner. Live-cell super-resolution imaging and biochemical in vivo proximity labeling demonstrated that Rab30-marked vesicles are highly dynamic and interact with proteins throughout the secretory pathway. Rab30 whole-body, liver-specific, and Rab30;Cpt2 liver-specific double knockout (DKO) mice are viable and display intact Golgi ultrastructure. However, the loss of Rab30 in Rab30;Cpt2 DKO mice suppresses serum dyslipidemia observed in Cpt2L-/- single knockout mice. Corresponding with decreased serum triglyceride and cholesterol levels, Rab30;Cpt2 DKO mice exhibit decreased circulating but not hepatic ApoA4 protein, indicative of a trafficking defect. Together, these data suggest a role for Rab30 in the selective sorting of lipoproteins to influence hepatocyte and circulating triglyceride levels particularly during times of excessive lipid burden.
HostingRepository	PRIDE
AnnounceDate	2023-09-12
AnnouncementXML	Submission_2023-09-12_11:01:04.878.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MichaelWolfgang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-12 20:59:12	ID requested
⏵ 1	2023-09-12 11:01:05	announced

Dataset with its publication pending

submitter keyword: lipoprotein, lipid metabolism, triglycerides, beta oxidation, vesicle transport,Ppara, Golgi apparatus, small GTPase

Michael J.Wolfgang
contact affiliation	Departments of Biological Chemistry and Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
contact email	mwolfga1@jhmi.edu
lab head
MichaelWolfgang
contact affiliation	Johns Hopkins University School of Medicine
contact email	mwolfga1@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044528
     1. Label: PRIDE project
     2. Name: Rab30 regulates lipid homeostasis in the hepatocyte during fasting