PXD044525-1

PXD044525 is an original dataset announced via ProteomeXchange.

Title	Eggs of the mosquito Aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism
Description	Upon water loss, some organisms pause their life cycles and escape death. While widespread in microbes, this is less common in animals. Aedes mosquitoes are vectors for viral diseases. Aedes eggs can survive dry environments, but molecular and cellular principles enabling egg survival through desiccation remain unknown. In this report, we find that Aedes aegypti eggs, in contrast to Anopheles stephensi, survive desiccation by acquiring desiccation tolerance at a late developmental stage. We uncover unique proteome and metabolic state changes in Aedes embryos during desiccation that reflect reduced central carbon metabolism, rewiring towards polyamine production, and enhanced lipid utilization for energy and polyamine synthesis. Using inhibitors targeting these processes in blood-fed mosquitoes that lay eggs, we infer a two-step process of desiccation tolerance in Aedes eggs. The metabolic rewiring towards lipid breakdown and dependent polyamine accumulation confers resistance to desiccation. Furthermore, rapid lipid breakdown is required to fuel energetic requirements upon water re-entry to enable larval hatching and survival upon rehydration. This study is fundamental to understanding Aedes embryo survival and in controlling the spread of these mosquitoes.
HostingRepository	PRIDE
AnnounceDate	2023-09-26
AnnouncementXML	Submission_2023-09-26_02:11:12.496.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SreesaSreedharan
SpeciesList	scientific name: Aedes aegypti aegypti; NCBI TaxID: 1424507;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-12 05:48:06	ID requested
⏵ 1	2023-09-26 02:11:12	announced
2	2024-10-22 06:03:53	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: lipid metabolism, regeneration, metabolic rewiring,Aedes mosquito, desiccation

SunilLaxman
contact affiliation	Associate Investigator, Metabolic Regulation of Cell Fate, inStem
contact email	sunil@instem.res.in
lab head
SreesaSreedharan
contact affiliation	Institue for Stem Cell Science and Regenerative Medicine
contact email	sreesas@instem.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD044525

PRIDE project URI

• PRIDE
  1. PXD044525
     1. Label: PRIDE project
     2. Name: Eggs of the mosquito Aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism