PXD044512 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A cytoplasmic chemoreceptor and Reactive Oxygen Species mediate bacterial chemotaxis to copper |
Description | Chemotaxis is a widespread strategy used by unicellular and multicellular living organisms to maintain their fitness in stressful environments. We previously showed that bacteria can trigger a negative chemotactic response to a copper (Cu)-rich environment. Cu ions toxicity on bacterial cell physiology has been mainly linked to mismetallation events and ROS production, although the precise role of Cu-generated ROS remains largely debated. Here, we found that the cytoplasmic Cu ions content mirrors variations of the extracellular Cu ions concentration and triggers a dose-dependent oxidative stress, which can be abrogated by superoxide dismutase and catalase overexpression. The inhibition of ROS production in the cytoplasm not only improves bacterial growth but also impedes Cu-chemotaxis, indicating that ROS derived from cytoplasmic Cu ions mediate the control of bacterial chemotaxis to Cu. We also identified the Cu chemoreceptor McpR, which binds Cu ions with low affinity, suggesting a labile interaction. In addition, we demonstrate that the cysteine 75 and histidine 99 within the McpR sensor domain are key residues in Cu chemotaxis and Cu coordination. Finally, we discovered that in vitro both Cu(I) and Cu(II) ions modulate McpR conformation in a distinct manner. Overall,our study provides mechanistic insights on a redox-based control of Cu chemotaxis, indicating that the cellular redox status can play a key role in bacterial chemotaxis. |
HostingRepository | PRIDE |
AnnounceDate | 2023-09-05 |
AnnouncementXML | Submission_2023-09-05_07:30:08.872.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD044512 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | MarcDieu |
SpeciesList | scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Caulobacter vibrioides; NCBI TaxID: 155892; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-11 07:14:45 | ID requested | |
⏵ 1 | 2023-09-05 07:30:09 | announced | |
2 | 2023-11-14 07:14:30 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: reactive oxygen species (ROS), stress response, bacteria,Chemotaxis, copper |
Contact List
Prof. Jean-YvesMATROULE |
contact affiliation | Research Unit in Microorganisms Biology (URBM), Narilis Institute, University of Namur, Namur, Belgium. |
contact email | jean-yves.matroule@unamur.be |
lab head | |
MarcDieu |
contact affiliation | University of Namur - MaSUN |
contact email | marc.dieu@unamur.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD044512 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044512
- Label: PRIDE project
- Name: A cytoplasmic chemoreceptor and Reactive Oxygen Species mediate bacterial chemotaxis to copper