PXD044487 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | BtcB (BP2265) is a T3SS chaperone of the CesT family in Bordetella pertussis |
Description | Bordetella pertussis is a Gram-negative, strictly human respiratory pathogen and the causative agent of whooping cough (pertussis). Similar to other Gram-negative pathogens, B. pertussis produces a functional type III secretion system, but its role in pathogenesis of B. pertussis is enigmatic and has not yet been elucidated. Here, we applied omics RNA-seq as well as LC-MS/MS techniques and co-immunoprecipitation method to identify and characterize the novel CesT family T3SS chaperone BP2265. Our results show that the chaperone BP2265 specifically interacts with the secreted T3SS anti-sigma factor BtrA. Moreover, in the absence of the chaperone, secretion but not production of BtrA and several early, intermediate, and late T3SS substrates is severely impaired. It appears that the role of BtrA in regulating T3SS is more complex and extends beyond its activity as an antagonist of the sigma factor BtrS. We propose to rename BP2265 as BtcB for the Bordetella type III chaperone of BtrA. |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-26 |
AnnouncementXML | Submission_2024-01-26_06:02:14.809.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Denisa Petrackova |
SpeciesList | scientific name: Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251); NCBI TaxID: 257313; scientific name: Bordetella pertussis B1917; NCBI TaxID: 743277; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-10 12:43:10 | ID requested | |
⏵ 1 | 2024-01-26 06:02:15 | announced | |
Publication List
10.1080/22221751.2023.2272638; |
Dr, ž, m, í, š, ek J, Petr, á, č, kov, á D, Dienstbier A, Č, urnov, á I, Ve, č, erek B, . Emerg Microbes Infect, 12(2):2272638(2023) [pubmed] |
Keyword List
submitter keyword: LC-MSMS, T3SS,Bordetella pertussis, RNA-seq |
Contact List
Branislav Vecerek, PhD |
contact affiliation | Czech Academy of Sciences Institute of Microbiology Laboratory of Post-Transcriptional Control of Gene Expression |
contact email | vecerek@biomed.cas.cz |
lab head | |
Denisa Petrackova |
contact affiliation | Institute of Microbiology of the CAS, v. v. i. |
contact email | petrack@biomed.cas.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044487
- Label: PRIDE project
- Name: BtcB (BP2265) is a T3SS chaperone of the CesT family in Bordetella pertussis