PXD044486-2

PXD044486 is an original dataset announced via ProteomeXchange.

Title	Stress Dependent Condensate Formation Regulated by the Ubiquitin Related Modifier Urm1
Description	The ability of proteins and RNA to coalesce into phase-separated assemblies is an emergent principle in organizing membraneless cellular compartments such as stress granules (SGs) and the nucleolus. While the individual constituents of these compartments have become increasingly well documented, the mechanisms underlying their rapid and reversible formation are only partially understood. Here, we report that the ubiquitin related modifier Urm1 functions in promoting the assembly of phase-separated compartments in yeast. Through its intrinsic ability to self-associate and interact with target proteins in a pH-sensitive manner, stress-induced Urm1 conjugation, mediated by the E1-like enzyme Uba4, drives efficient deposition of target proteins into phase-separated assemblies, including SGs and the nucleolus. Yeast cells lacking urmylation exhibit condensate assembly defects, causing reduced fitness upon exposure to stress-induced challenges. We propose that Urm1 acts as a reversible molecular “adhesive” to drive condensate formation of a wide range of proteins under various stress conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:47:53.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lucas Cairo
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-10 11:31:26	ID requested
1	2024-06-28 08:26:38	announced
⏵ 2	2024-10-22 06:47:53	announced	2024-10-22: Updated project metadata.

10.1016/J.CELL.2024.06.009;

submitter keyword: yeast URM1

F., Ulrich, Hartl
contact affiliation	Max-Planck-Insitute for Biochemistry, Department of cellular biochemistry
contact email	uhartl@biochem.mpg.de
lab head
Lucas Cairo
contact affiliation	Max-Planck-Insitute of Biochemistry
contact email	cairo@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD044486

PRIDE project URI

• PRIDE
  1. PXD044486
     1. Label: PRIDE project
     2. Name: Stress Dependent Condensate Formation Regulated by the Ubiquitin Related Modifier Urm1