PXD044475-1

PXD044475 is an original dataset announced via ProteomeXchange.

Title	Selective Regulation of Macrophage Lipid Metabolism via Nanomaterials’ Surface Chemistry
Description	Understanding the interfacial structure between nanomaterials and lipoproteins is crucial for gaining profound insights into how nanomaterials impact lipoprotein structure and interfere with lipid metabolism. In this study, we employed graphene oxide (GO) nanosheets (NSs) with precisely controlled surface hydrophilicity as model nanomaterials to investigate the influence of surface properties on lipoprotein corona formation. Our findings reveal that hydrophobic GOs exhibit a greater affinity for binding with low-density lipoprotein (LDL). To explore the interaction between GOs and LDL at the interface, we employed advanced techniques such as X-ray reflectivity, circular dichroism, and molecular simulation. Specifically, hydrophobic GOs showed a preference for associating with the lipid components of LDL, while hydrophilic ones tended to bind with apolipoproteins. Furthermore, our research demonstrated that these GOs distinctly modulate various lipid metabolism pathways, including LDL recognition, uptake, hydrolysis, efflux, and lipid droplet formation. This comprehensive investigation underscores the significance of structural analysis at the nano-biomolecule interface and emphasizes the critical role of nanomaterial’s surface properties in mediating cellular lipid metabolism. Our findings carry profound implications and can serve as a source of inspiration for the future design of biocompatible nanomaterials and nanomedicines.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_07:40:27.431.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liming Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-09 21:31:14	ID requested
⏵ 1	2025-05-06 07:40:28	announced

Wu J, Bai X, Yan L, Baimanov D, Cong Y, Quan P, Cai R, Guan Y, Bu W, Lin B, Wang J, Yu S, Li S, Chong Y, Li Y, Hu G, Zhao Y, Chen C, Wang L, Selective regulation of macrophage lipid metabolism via nanomaterials' surface chemistry. Nat Commun, 15(1):8349(2024) [pubmed]

10.1038/s41467-024-52609-7;

submitter keyword: Human plasma, Low density lipoprotein, LC-MS, Lipid metabolism,Graphene oxide, Surface hydrophilicity

Chunying Chen
contact affiliation	CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics and National Center for Nanoscience and Technology of China, Chinese Academy of Sciences, Beijing 100049, P. R. China
contact email	chenchy@nanoctr.cn
lab head
Liming Wang
contact affiliation	Institute of High Energy Physics, Chinese Academy of Sciences
contact email	wangliming@ihep.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD044475

PRIDE project URI

• PRIDE
  1. PXD044475
     1. Label: PRIDE project
     2. Name: Selective Regulation of Macrophage Lipid Metabolism via Nanomaterials’ Surface Chemistry